Bioprinting-assisted tissue assembly to generate organ substitutes at scale

• Published in: Trends in biotechnology (Regular ed.) Vol. 41; no. 1; pp. 93 - 105
• Main Authors: Jo, Yeonggwon, Hwang, Dong Gyu, Kim, Myungji, Yong, Uijung, Jang, Jinah
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.01.2023; Elsevier Limited
• Subjects: 3-D printers; 3D bioprinting; Aggregates; Architecture; Artificial Intelligence; Assembly; Automation; Biocompatible Materials; Bioengineering; biomaterial; Biomaterials; Biomedical materials; Bioprinting; Cancer; Design; Hydrogels; Manufacturing; Morphogenesis; Physiology; Printing, Three-Dimensional; Robotics; Standardization; Three dimensional printing; tissue assembly; Tissue Engineering; Tissue Scaffolds; vascularization; tissue assembly; tissue engineering; 3D bioprinting; vascularization; biomaterial
• ISSN: 0167-7799; 1879-3096
• DOI: 10.1016/j.tibtech.2022.07.001

Various external cues can guide cellular behavior and maturation during developmental processes. Recent studies on bioprinting-assisted tissue engineering have considered this a practical, versatile, and flexible way to provide external cues to developing engineered tissues. An ensemble of multiple external cues can improve the speed and capability of morphogenesis. In this review, we discuss how bioprinting and biomaterials provide multiple guidance to generate micro-sized building blocks with specific shapes and also highlight their applications in tissue assembly toward volumetric tissue and organ generation. Furthermore, we discuss our perspectives on the future translation of bioprinting technologies integrated with artificial intelligence (AI) and robot-assisted apparatus to promote automation, standardization, and clinical translation of bioprinted tissues. 3D bioprinting, which is a representative approach of tissue engineering, can provide structural cues by arranging cells in a specific shape.The development of 3D bioprinting techniques has enabled the fabrication of various shapes, such as points, lines, and planes, on the microscale.3D-bioprinted tissues recapitulate the unique structure of native tissues, promoting the appropriate function of such tissues.The fabrication of volumetric and complex tissues can be achieved by controlling the mechanical features of bioinks and by using an assembly approach.