Clavulanic acid biosynthesis in Streptomyces clavuligerus: gene cloning and characterization

Seven classes of Streptomyces clavuligerus mutants defective in clavulanic acid (CLA) biosynthesis have been identified and used to clone the chromosomal DNA encoding eight CLA biosynthetic genes. The complete sequences of three and the partial sequences of two of these biosynthetic genes are report...

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Published inGene Vol. 166; no. 1; pp. 49 - 55
Main Authors Hodgson, John E, Fosberry, Andrew P, Rawlinson, Nichola S, Ross, Hamish N.M, Neal, Robert J, Arnell, Janice C, Earl, Alison J, Lawlor, Elizabeth J
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.12.1995
Subjects
Acetyltransferase
Acetyltransferases - genetics
amidinohydrolase
Amino Acid Sequence
Anti-Bacterial Agents - biosynthesis
antibiotic
arginine
Base Sequence
Clavulanic Acid
Clavulanic Acids - biosynthesis
Cloning, Molecular
DNA, Bacterial - genetics
Genes, Bacterial
Genetic Complementation Test
Molecular Sequence Data
phosphate
Promoter Regions, Genetic
Restriction Mapping
Streptococcus - genetics
transport
Ureohydrolases - genetics
β-lactam
antibiotic
arg box
B
ORF
cs2
OAT
amidinohydrolase
nt
dcl
bp
arginine
AUH
kb
pI
Acetyltransferase
HPLC
aa
RBS
SSC
SDS
phosphate
transport
β-lactam
PHOB
Sc
CAS
PAH
OPPA
CLA
pho box
Ec
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Summary:Seven classes of Streptomyces clavuligerus mutants defective in clavulanic acid (CLA) biosynthesis have been identified and used to clone the chromosomal DNA encoding eight CLA biosynthetic genes. The complete sequences of three and the partial sequences of two of these biosynthetic genes are reported, together with their known or predicted functions.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(95)00560-9