Biocompatibility of marine magnetotactic ovoid strain MO-1 for in vivo application

• Published in: Journal of oceanology and limnology Vol. 39; no. 6; pp. 2107 - 2115
• Main Authors: Chen, Changyou, Wang, Pingping, Wu, Long-Fei, Song, Tao
• Format: Journal Article
• Language: English
• Published: Heidelberg Science Press 01.11.2021; Springer Nature B.V; Aix Marseille University,CNRS,LCB,Marseille F-13402,France%Beijing Key Laboratory of Bioelectromagnetism,Institute of Electrical Engineering,Chinese Academy of Sciences,Beijing 100190,China; Beijing Key Laboratory of Bioelectromagnetism,Institute of Electrical Engineering,Chinese Academy of Sciences,Beijing 100190,China; France-China Joint Laboratory for Evolution and Development of Magnetotactic Multicellular Organisms,Chinese Academy of Sciences,Beijing 100029,China%France-China Joint Laboratory for Evolution and Development of Magnetotactic Multicellular Organisms,Chinese Academy of Sciences,Beijing 100029,China; France-China Joint Laboratory for Evolution and Development of Magnetotactic Multicellular Organisms,Chinese Academy of Sciences,Beijing 100029,China; University of the Chinese Academy of Sciences,Beijing 100049,China
• Subjects: Antibiotic resistance; Bacteria; Biocompatibility; Biomedical materials; Blood plasma; Cell viability; Cells; Earth and Environmental Science; Earth Sciences; Lethal dose; Magnetic field; Magnetic fields; Magnetotaxis; Microbiological strains; Microrobots; Nanoparticles; Oceanography; Pathogens; Swimming; Toxicity; motility; magnetotactic bacteria; cell interaction; median lethal dose
• ISSN: 2096-5508; 2523-3521
• DOI: 10.1007/s00343-021-0420-7

Magnetotactic bacteria are capable of biosynthesizing magnetic nanoparticles, also called magnetosomes, and swimming along magnetic field lines. The abilities endow the whole cells of magnetotactic bacteria with such applications as targeted therapy and manipulation of microrobots. We have shown that the intact marine magnetotactic bacteria MO-1 kill efficiently antibiotic-resistant pathogen Staphylococcus aureus in vivo, but the biocompatibility of this marine bacterium is unknown. In this study, the strain MO-1 was chosen to analyze its biocompatibility and potential for biomedicine applications. Results showed that MO-1 cells could be guided at 37 °C under an external magnetic field and swim in the blood plasma and urine. They could keep active locomotivity within 40 min in the plasma and urine, although their velocity slowed down. When incubated with human cells, magnetotactic bacteria MO-1 had no obvious effects on cellular viability at low dose, while the cell toxicity increased with the augmentation of the quantity of the MO-1 cells added. In the in-vivo experiments, the median lethal dose of magnetotactic bacteria MO-1 in rats was determined to be 7.9×10 10 bacteria/kg. These results provided the foundation for the biocompatibility and safety evaluations of magnetotactic bacteria MO-1 and suggested that they could be basically used in clinical targeted therapy.