Determination of Rutin in Amaranthus spinosus Linn. Whole Plant Powder by HPTLC

• Published in: Chromatographia Vol. 65; no. 11-12; pp. 767 - 769
• Main Authors: Suryavanshi, V. L, Sathe, P. A, Baing, M. M, Singh, G. R, Lakshmi, S. N
• Format: Journal Article
• Language: English
• Published: Oxford Wiesbaden : Vieweg Verlag 01.06.2007; Springer; Springer Nature B.V
• Subjects: Amaranthus spinosus Linn; Biological and medical sciences; Carcinogens; Chromatography; Densitometers; Distilled water; Ethyl acetate; Formic acid; General pharmacology; Medical sciences; Methanol; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; rutin; Silica gel; thin layer chromatography; Validation; Chemical analysis; Pharmacognosy; Whole plant; Amaranthaceae; Rutin; Flavonoid; Powder; Medicinal plant; Thin layer chromatography; Polyphenol; Ultraviolet detector; Amaranthus spinosus Linn; Dicotyledones; Angiospermae; Plant origin; Flavone derivatives; Thin-layer chromatography; Herbaceous plant; Spermatophyta; Glycoside; Densitometry; Quantitative analysis
• ISSN: 0009-5893; 1612-1112
• DOI: 10.1365/s10337-007-0239-1

A simple, precise and accurate high-performance thin-layer chromatographic method has been established for the determination of rutin in the whole plant powder of Amaranthus spinosus Linn. Rutin has been reported to have anti-diabetic, anti-thrombotic, anti-inflammatory and anti-carcinogenic activity. A methanol extract of the whole plant powder was used for the experimental work. The concentration of rutin in the whole plant powder was found to be 0.15%. Separation was performed on silica gel 60 F₂₅₄ HPTLC plates with ethyl acetate:formic acid:methanol:distilled water in the proportion 10:0.9:1.1:1.7 (v/v), as mobile phase. The determination was carried out using the densitometric absorbance mode at 363 nm. Rutin response was linear over the range 10-60 μg mL-¹. The HPTLC method was evaluated in terms of sensitivity, accuracy, precision and reproducibility.