Cloning One CIPK Gene from a Thermo-Sensitive Genic Self-Incompatible Line in Maize Expressing Under Different Temperatures

A calcineurin B-like protein-interacting protein kinases （CIPK） gene was cloned （EU 424058） from a thermo-sensitive genic self-incompatibility （TSGI） line, HE97, in maize, which was selected for suppression subtractive hybridization （SSH） library of the self-incompatible and self-compatible silks of...

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Published inAgricultural sciences in China Vol. 10; no. 6; pp. 813 - 819
Main Authors LIN, Xing-e, JI, Hong-qing, NIU, Jun-hai, HU, Yan-min, FU, Zhong-jun, LIU, Zong-hua, TANG, Ji-hua
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.06.2011
College of Agronomy, Henan Agricultural University, Zhengzhou 450002, P.R. China
Subjects
calcium
CIPK gene
cloning
complementary DNA
corn
expression
genes
maize
nucleotides
open reading frames
PK基因
pollen
pollen tubes
protein kinases
quantitative polymerase chain reaction
second messengers
suppression subtractive hybridization
temperature
克隆
抑制性消减杂交
温度
温敏核
玉米
自交不亲和性
钙调神经磷酸酶
temperature
expression
CIPK gene
maize
cloning
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Summary:A calcineurin B-like protein-interacting protein kinases （CIPK） gene was cloned （EU 424058） from a thermo-sensitive genic self-incompatibility （TSGI） line, HE97, in maize, which was selected for suppression subtractive hybridization （SSH） library of the self-incompatible and self-compatible silks of the TSGI line under different temperatures. The full-length cDNA of the candidate CIPK-like gene consisted of 1 918 nucleotides and contained a 1 332-bp open reading frame in maize. Real-time PCR indicated that the candidate CIPK-like gene was highly expressed in the self-incompatible pollen of the TGSI line, and promoted elevated levels of calcium （Ca2＋）. High Ca2＋ concentrations in the pollen strongly inhibit pollen tube formation in silk of the same plant, thus inducing self-incompatibility （SI） under high temperature. These results implied that the CIPKs-like gene would play an important role in the regulation of HE97 characteristics under different temperatures, perhaps acting as a secondary messenger in the expression of SI in the TGSI line in maize.
Bibliography:CIPK gene, cloning, expression, temperature, maize
A calcineurin B-like protein-interacting protein kinases （CIPK） gene was cloned （EU 424058） from a thermo-sensitive genic self-incompatibility （TSGI） line, HE97, in maize, which was selected for suppression subtractive hybridization （SSH） library of the self-incompatible and self-compatible silks of the TSGI line under different temperatures. The full-length cDNA of the candidate CIPK-like gene consisted of 1 918 nucleotides and contained a 1 332-bp open reading frame in maize. Real-time PCR indicated that the candidate CIPK-like gene was highly expressed in the self-incompatible pollen of the TGSI line, and promoted elevated levels of calcium （Ca2＋）. High Ca2＋ concentrations in the pollen strongly inhibit pollen tube formation in silk of the same plant, thus inducing self-incompatibility （SI） under high temperature. These results implied that the CIPKs-like gene would play an important role in the regulation of HE97 characteristics under different temperatures, perhaps acting as a secondary messenger in the expression of SI in the TGSI line in maize.
11-4720/S
http://dx.doi.org/10.1016/S1671-2927(11)60066-7
ISSN:1671-2927
DOI:10.1016/S1671-2927(11)60066-7