Calcium alginate microcapsules with spherical liquid cores templated by gelatin microparticles for mass production of multicellular spheroids

Multicellular spheroids are important in biomedical applications, such as drug research and regenerative medicine. We developed microcapsules from sodium alginate and gelatin for mass production of size-controlled spheroids with diameters <200μm. The microcapsules were composed of calcium alginat...

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Bibliographic Details
Published inActa biomaterialia Vol. 6; no. 8; pp. 3132 - 3137
Main Authors Sakai, Shinji, Ito, Sho, Kawakami, Koei
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.08.2010
Subjects
Alginate
Alginates - chemical synthesis
Alginates - pharmacology
Animals
Capsules
Cats
Cell Culture Techniques - methods
Cell encapsulation
Cell Line
Cell Proliferation - drug effects
Fluorescein-5-isothiocyanate - metabolism
Gelatin
Gelatin - chemical synthesis
Gelatin - chemistry
Glucuronic Acid - chemical synthesis
Glucuronic Acid - pharmacology
Hexuronic Acids - chemical synthesis
Hexuronic Acids - pharmacology
Microcapsule
Microencapsulation
Microspheres
Spheroids, Cellular - cytology
Spheroids, Cellular - drug effects
Alginate
Microencapsulation
Gelatin
Cell encapsulation
Microcapsule
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Summary:Multicellular spheroids are important in biomedical applications, such as drug research and regenerative medicine. We developed microcapsules from sodium alginate and gelatin for mass production of size-controlled spheroids with diameters <200μm. The microcapsules were composed of calcium alginate gels with spherical liquid cores (diameter≈150μm) for formation of spheroids. The spherical liquid cores were prepared by incubating calcium alginate microcapsules containing thermally gelled, cell-enclosing gelatin microparticles about 150μm in diameter, at 37°C. The gelatin microparticles were encapsulated within the microcapsules by dropping a sodium alginate solution containing suspended gelatin microparticles into 100mM CaCl2. The enclosed feline renal fibroblast cell line, CRFK, cells showed 93.8% viability immediately after encapsulation, then grew and completely filled the spherical cores. Multicellular spheroids were collected within 1min by soaking microcapsules in a medium containing alginate lyase.
Bibliography:ObjectType-Article-1
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ISSN:1742-7061
1878-7568
DOI:10.1016/j.actbio.2010.02.003