Oxygen consumption and blood flow distribution in perfused skeletal muscle of chinook salmon

• Published in: Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology Vol. 179; no. 3; pp. 359 - 368
• Main Authors: Forgan, Leonard G, Forster, Malcolm E
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.04.2009; Springer-Verlag; Springer Nature B.V
• Subjects: Analysis of Variance; Animal Physiology; Animals; Biochemistry; Biomedical and Life Sciences; Biomedicine; Blood Pressure; Electric Stimulation; Exercise; Human Physiology; Life Sciences; Metabolism; Microspheres; Mitochondria - metabolism; Muscle, Skeletal - blood supply; Musculoskeletal system; New Zealand; Original Paper; Oxygen - metabolism; Oxygen Consumption - physiology; Physical fitness; Physiology; Regional Blood Flow; Salmon - physiology; Tail - blood supply; Tail - physiology; Tetrazolium Salts; Thiazoles; Zoology; New Zealand; Oxygen consumption; Salmon; Perfusion; Skeletal muscle; Blood flow
• ISSN: 0174-1578; 1432-136X
• DOI: 10.1007/s00360-008-0320-6

An isolated, perfused salmon tail preparation showed oxyconformance at low oxygen delivery rates. Addition of pig red blood cells to the perfusing solution at a haematocrit of 5 or 10% allowed the tail tissues to oxyregulate. Below ca. 60 ml O₂ kg⁻¹ h⁻¹ of oxygen delivery (DO₂), VO₂ was delivery dependent. Above this value additional oxygen delivery did not increase VO₂ of resting muscle above ca. 35 ml O₂ kg⁻¹ h⁻¹. Following electrical stimulation, VO₂ increased to ca. 65 ml O₂ kg⁻¹ h⁻¹, with a critical DO₂ of ca. 150 ml O₂ kg⁻¹ h⁻¹. Dorsal aortic pressure fell to 69% of the pre-stimulation value after 5 min of stimulation and to 54% after 10 min. Microspheres were used to determine blood flow distribution (BFD) to red (RM) and white muscle (WM) within the perfused myotome. Mass specific BFD ratio at rest was found to be 4.03 ± 0.49 (RM:WM). After 5 min of electrical stimulation the ratio did not change. Perfusion with saline containing the tetrazolium salt 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) revealed significantly more mitochondrial activity in RM. Formazan production from MTT was directly proportional to time of perfusion in both red and WM. The mitochondrial activity ratio (RM:WM) did not change over 90 min of perfusion.