Integration host factor is important for biofilm formation by Salmonella enterica Enteritidis

• Published in: Pathogens and disease Vol. 75; no. 6
• Main Authors: Leite, Bruna, Werle, Catierine Hirsch, Carmo, Camila Pinheiro do, Nóbrega, Diego Borin, Milanez, Guilherme Paier, Culler, Hebert Fabricio, Sircili, Marcelo Palma, Alvarez-Martinez, Cristina E., Brocchi, Marcelo
• Format: Journal Article
• Language: English
• Published: United States Oxford University Press 31.08.2017
• Subjects: Bacteria; Biofilms; Biofilms - growth & development; Cellulose; Cellulose - biosynthesis; Clonal deletion; Eggs; Fimbriae, Bacterial - genetics; Fimbriae, Bacterial - metabolism; Food contamination; Gene Deletion; Gene Expression Regulation, Bacterial; Genetic Fitness; Genotype & phenotype; Integration; Integration host factor; Integration Host Factors - deficiency; Integration Host Factors - genetics; Microbiology; Pellicle; Pili; Plankton - genetics; Plankton - growth & development; Plankton - metabolism; Planktonic cells; Polysaccharides, Bacterial - biosynthesis; Polysaccharides, Bacterial - deficiency; Poultry; Protein Subunits - deficiency; Protein Subunits - genetics; Public health; Salmonella; Salmonella enterica; Salmonella enteritidis - genetics; Salmonella enteritidis - growth & development; Salmonella enteritidis - metabolism; Salmonella enteritidis - pathogenicity; Viability; polysaccharide matrix; Enteritidis; curli fimbriae; cellulose; integration host factor; biofilm; Salmonella enterica Enteritidis
• ISSN: 2049-632X; 2049-632X
• DOI: 10.1093/femspd/ftx074

Abstract Salmonella enterica Enteritidis forms biofilms and survives in agricultural environments, infecting poultry and eggs. Bacteria in biofilms are difficult to eradicate compared to planktonic cells, causing serious problems in industry and public health. In this study, we evaluated the role of ihfA and ihfB in biofilm formation by S. enterica Enteritidis by employing different microbiology techniques. Our data indicate that ihf mutant strains are impaired in biofilm formation, showing a reduction in matrix formation and a decrease in viability and metabolic activity. Phenotypic analysis also showed that deletion of ihf causes a deficiency in curli fimbriae expression, cellulose production and pellicle formation. These results show that integration host factor has an important regulatory role in biofilm formation by S. enterica Enteritidis. Integration host factor subunit roles in Salmonella enterica Enteritidis biofilm formation.