Characterization of the genetic background of Vibrio cholerae O1 biotype El Tor serotype Inaba strains isolated in Trivandrum, southern India

• Published in: Journal of medical microbiology Vol. 56; no. 2; pp. 260 - 265
• Main Authors: Mohapatra, Saswat S, Ramachandran, Dhanya, Mantri, Chinmay K, Singh, Durg V
• Format: Journal Article
• Language: English
• Published: England Soc General Microbiol 01.02.2007
• Subjects: Anti-Bacterial Agents - pharmacology; Cholera - epidemiology; Cholera - microbiology; Cholera Toxin - genetics; Disease Outbreaks; DNA, Bacterial - genetics; Drug Resistance, Bacterial; Gene Dosage; Genes, Bacterial; Genomic Islands - genetics; Humans; India - epidemiology; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Ribotyping; Serotyping; Vibrio cholerae; Vibrio cholerae O1 - classification; Vibrio cholerae O1 - drug effects; Vibrio cholerae O1 - genetics; Vibrio cholerae O1 - isolation & purification; India
• ISSN: 0022-2615; 1473-5644
• DOI: 10.1099/jmm.0.46868-0

1 Institute of Life Sciences, Nalco Square, Bhubaneswar 751 023, India 2 Rajiv Gandhi Centre for Biotechnology, Jagathy, Trivandrum 695 014, India Correspondence Durg V. Singh durg_singh{at}yahoo.co.in Received 2 August 2006 Accepted 18 October 2006 Isolates of Vibrio cholerae O1 biotype El Tor serotype Inaba associated with an outbreak of cholera in Trivandrum, southern India, were characterized. PCR testing revealed that all five isolates examined carried the TCP pathogenicity island, the CTX genetic element and the RTX toxin, and produced cholera toxin (CT). RFLP analysis revealed that these Inaba isolates possessed a single copy of the CTX element flanked by two tandemly arranged copies of the RS element upstream of the core region. The isolates were resistant to ampicillin, nalidixic acid, trimethoprim, sulfamethoxazole, streptomycin and the vibriostatic agent 2,4-diamino-6,7-diisopropylpteridine (O/129). Ribotyping of these Inaba isolates revealed a hybridization profile similar to a strain of serotype Ogawa prevalent in southern India. Abbreviations: CT, cholera toxin.