Comparison of doubly labeled water, intake-balance, and direct- and indirect-calorimetry methods for measuring energy expenditure in adult men

• Published in: The American journal of clinical nutrition Vol. 52; no. 1; pp. 66 - 71
• Main Authors: Seale, JL, Rumpler, WV, Conway, JM, Miles, CW
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 01.07.1990; American Society for Clinical Nutrition
• Subjects: Activities of Daily Living; Adult; ADULTS; AGE GROUPS; ANIMALS; balance studies; BASIC BIOLOGICAL SCIENCES; Biological and medical sciences; BODY COMPOSITION; Body Water; Body Water - metabolism; Body Weight; CALORIFIC VALUE; CALORIMETRY; Calorimetry, Indirect; COMBUSTION PROPERTIES; COMPARATIVE EVALUATIONS; DEUTERIUM; DOUBLE LABELLING; energy expenditure; Energy Intake; Energy Intake - physiology; Energy Metabolism; Energy Metabolism - physiology; EVEN-EVEN NUCLEI; heat production; Humans; HYDROGEN COMPOUNDS; HYDROGEN ISOTOPES; Investigative techniques, diagnostic techniques (general aspects); ISOTOPE APPLICATIONS; ISOTOPES; LABELLING; LIGHT NUCLEI; Male; MAMMALS; MAN; measurement; Medical sciences; men; Metabolic diseases; METABOLISM; Middle Aged; NUCLEI; ODD-ODD NUCLEI; OXYGEN 18; OXYGEN COMPOUNDS; OXYGEN ISOTOPES; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; physiology; PRIMATES; Radioisotope Dilution Technique; respiration; STABLE ISOTOPES; T200; TRACER TECHNIQUES; VERTEBRATES 550501 > Metabolism > Tracer Techniques; WATER; WEIGHT; Water; Human; Energy metabolism; Double labelling; Exploration; Technique; Comparative study
• ISSN: 0002-9165; 1938-3207
• DOI: 10.1093/ajcn/52.1.66

Energy expenditure (EE) of four adult men on a weight-maintenance diet was estimated by use of doubly labeled water, intake balance, and direct and indirect calorimetry. The doubly labeled water (2H218O) method was used to estimate free-living EE for 13 d. Metabolizable energy (ME) intake was used to estimate free-living EE for 1 wk. The subjects’ 24-h EE was measured in a dual direct-indirect room calorimeter on 3 alternate days. Estimates of free-living EE as measured by ME intake and doubly labeled water indicate agreement between the two methods (mean difference +/- SEM, -1.04 +/- 0.63%). Measurements of EE with indirect and direct calorimetry are equivalent (mean difference 0.63 +/- 0.44%). The daily EE measured by doubly labeled water in these free-living adults over a 13-d period was 15.01% greater than the 24-h EE measured within the calorimeter.