Determination of oxidative status and apoptosis in peripheral blood of dogs with sarcoptic mange

• Published in: Veterinary parasitology Vol. 178; no. 3; pp. 330 - 338
• Main Authors: Singh, S.K., Dimri, U., Sharma, M.C., Swarup, D., Sharma, B.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 10.06.2011
• Subjects: Animals; Apoptosis; Apoptosis - physiology; Blood Cell Count; Canis; Catalase - blood; Dog Diseases - blood; Dog Diseases - enzymology; Dog Diseases - parasitology; Dog Diseases - pathology; Dogs; Flow Cytometry; Glutathione - blood; Glutathione Peroxidase - blood; Glutathione Transferase - blood; Malondialdehyde - blood; Mange; Membrane Potential, Mitochondrial - physiology; Oxidant/antioxidant; Oxidative Stress - physiology; Sarcoptes scabiei; Sarcoptes scabiei - physiology; Scabies - blood; Scabies - parasitology; Scabies - pathology; Scabies - veterinary; Superoxide Dismutase - blood; Oxidant/antioxidant; Mange; Sarcoptes scabiei; Dogs; Apoptosis
• ISSN: 0304-4017; 1873-2550
• DOI: 10.1016/j.vetpar.2011.01.036

The aim of the present study was to determine the erythrocytic oxidant/antioxidant balance and apoptosis of peripheral blood leukocytes of dogs with natural Sarcoptes scabiei var. canis mite infestation. A total of twenty four clinically Sarcoptes-infested dogs were examined and used to execute the study. While another twenty four healthy dogs free of any ecto-parasite were used as controls. Peripheral blood samples were obtained from each infested only once on the day of dermatological examinations. Determination of oxidant/antioxidant balance was conceded by estimating the levels of lipid peroxides and antioxidants in erythrocytes. While, apoptosis of peripheral blood leukocytes was determined by estimating externalization of phosphatidylserine (PS) at the cell surface as well as by detection of depolarization mitochondrial membrane potential (Δ Ψm) by flow cytometry. Sarcoptes-infested dogs had revealed significantly higher ( P ≤ 0.001) contents of erythrocytic lipid peroxides in comparison with the healthy controls. Whereas the level of reduced glutathione was found to be significantly lower ( P ≤ 0.001) in Sarcoptes-infested dogs as compared to the healthy dogs. The activity of glutathione peroxidase was found to be significantly lower ( P ≤ 0.001) in Sarcoptes-infested dogs as compared to the healthy dogs. The activity of glutathione-S-transferase was also found to be significantly lower ( P ≤ 0.001) in Sarcoptes-infested dogs as compared to the healthy dogs. The dogs with sarcoptic mange had revealed significantly lower ( P ≤ 0.001) activity of superoxide dismutase in coparision with the healthy dogs. The dogs with sarcoptic mange had also revealed significantly lower ( P ≤ 0.001) activity of catalase in coparision with the healthy dogs. The percentage of apoptotic leukocytes was found to be significantly higher ( P ≤ 0.001) in Sarcoptes-infested dogs as compared to the healthy controls. Sarcoptes-infested dogs had also exhibited significantly ( P ≤ 0.001) higher percentage of leukocytes with depolarized mitochondrial membrane potential in comparison with the healthy controls. It is concluded that significant alteration in oxidant/antioxidant balance and increased rate of apoptosis in peripheral leukocytes may be implicated in the pathogenesis of clinical Sarcoptes mite infestation in dogs.