Enhanced healing of rabbit segmental radius defects with surface-coated calcium phosphate cement/bone morphogenetic protein-2 scaffolds

• Published in: Materials Science & Engineering C Vol. 44; pp. 326 - 335
• Main Authors: Wu, Yi, Hou, Juan, Yin, ManLi, Wang, Jing, Liu, ChangSheng
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2014
• Subjects: Animals; Bone Cements - chemistry; Bone Cements - pharmacology; Bone Morphogenetic Protein 2 - pharmacology; Bone morphogenetic protein-2; Bone regeneration; Bone Regeneration - drug effects; Calcium phosphate cement; Calcium Phosphates - chemistry; Carboxymethylcellulose Sodium - chemistry; Carboxymethylcellulose Sodium - pharmacology; Cellulose; Coated Materials, Biocompatible - chemistry; Hypromellose Derivatives - chemistry; Hypromellose Derivatives - pharmacology; Male; Mice; Polyvinyl Alcohol - chemistry; Polyvinyl Alcohol - pharmacology; Rabbits; Radius - drug effects; Surface Properties; Tissue Scaffolds - chemistry; Wound Healing - drug effects; Bone morphogenetic protein-2; Calcium phosphate cement; Bone regeneration; Cellulose
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2014.08.020

Large osseous defects remain a difficult clinical problem in orthopedic surgery owing to the limited effective therapeutic options, and bone morphogenetic protein-2 (BMP-2) is useful for its potent osteoinductive properties in bone regeneration. Here we build a strategy to achieve prolonged duration time and help inducting new bone formation by using water-soluble polymers as a protective film. In this study, calcium phosphate cement (CPC) scaffolds were prepared as the matrix and combined with sodium carboxymethyl cellulose (CMC-Na), hydroxypropylmethyl cellulose (HPMC), and polyvinyl alcohol (PVA) respectively to protect from the digestion of rhBMP-2. After being implanted in the mouse thigh muscles, the surface-modified composite scaffolds evidently induced ectopic bone formation. In addition, we further evaluated the in vivo effects of surface-modified scaffolds in a rabbit radius critical defect by radiography, three dimensional micro-computed tomographic (μCT) imaging, synchrotron radiation-based micro-computed tomographic (SRμCT) imaging, histological analysis, and biomechanical measurement. The HPMC-modified CPC scaffold was regarded as the best combination for segmental bone regeneration in rabbit radius. •A simple surface-coating method was used to fabricate composite scaffolds.•Growth factor was protected from rapid depletion via superficial coating.•Significant promotion of bone regeneration was achieved.•HPMC-modification displayed optimal effect of bone regeneration.