Unique features of Erwinia chrysanthemi ( Dickeya dadantii) RA3B genes involved in the blue indigoidine production
Erwinia chrysanthemi (Ech) RA3B produces a large amount of blue indigoidine. Using Tn 5-induced mutagenesis, three indigoidine-deficient mutants were generated. Followed by library screening, a 5.8 kb fragment complemented mutants for indigoidine synthesis was cloned. This fragment contains four com...
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Published in | Microbiological research Vol. 165; no. 6; pp. 483 - 495 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Germany
Elsevier GmbH
20.08.2010
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Subjects | |
Online Access | Get full text |
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Summary: | Erwinia chrysanthemi (Ech) RA3B produces a large amount of blue indigoidine. Using Tn
5-induced mutagenesis, three indigoidine-deficient mutants were generated. Followed by library screening, a 5.8
kb fragment complemented mutants for indigoidine synthesis was cloned. This fragment contains four complete open-reading frames (ORFs),
pecS,
pecM,
idgA, and
idgB, and two partial ORFs,
argG, and
idgC. These genes are nearly identical to those in strain Ech3937. Primer extension assays demonstrated a clear transcriptional start site prior to
idgA, while no promoter preceding
idgB and
idgC was detected, suggesting that
idgA,
idgB, and
idgC are organized as one transcription unit. In contrast,
indAB is separated from
indC in Ech3937. Interestingly, an ERIC sequence was present between
idgB and
idgC in place of the promoter region of the homolog
indC, which may contribute to the loss of promoter activity in RA3B. Futhermore,
idgB mutant displayed much lighter blue color, while
indB mutant appeared white on media. Overexpression of
pecS in RA3B resulted in significantly reduced indigoidine production and
idgC transcript. Moreover, gel shift and
luxAB reporter assays revealed that PecS specifically binds to the sequence preceding
idgA and inhibits gene expression, which is consistent with the results observed in Ech3937. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0944-5013 1618-0623 |
DOI: | 10.1016/j.micres.2009.09.004 |