Isolation of proteolytic bacteria from mealworm (Tenebrio molitor) exoskeletons to produce chitinous material

• Published in: FEMS microbiology letters Vol. 364; no. 17
• Main Authors: da Silva, Fernanda Kerche Paes, Brück, Dieter W., Brück, Wolfram M.
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 15.09.2017
• Subjects: Acid production; Animal Shells - microbiology; Animals; Bacteria; Bacteria - enzymology; Bacteria - isolation & purification; Bacteria - metabolism; Chitin; Chitin - chemistry; Chitin - isolation & purification; Chitin - metabolism; Exoskeleton; Exoskeletons; Fermentation; Fourier analysis; Infrared analysis; Infrared spectroscopy; Insects; Lactobacillus plantarum; Lactobacillus plantarum - enzymology; Lactobacillus plantarum - isolation & purification; Lactobacillus plantarum - metabolism; Larva - microbiology; Microbiology; Microorganisms; Peptide Hydrolases - biosynthesis; Peptide Hydrolases - metabolism; Population growth; Protein sources; Proteins; Proteins - metabolism; Proteolysis; Proteolytic bacteria; Reflectance; Spectrophotometry, Infrared; Tenebrio - microbiology; Tenebrio molitor; fermentation; exoskeleton; screening; chitin; mealworms; bacterial isolation
• ISSN: 1574-6968; 0378-1097; 1574-6968
• DOI: 10.1093/femsle/fnx177

Abstract The use of insects as a source of protein is becoming an important factor for feeding an increasing population. After protein extraction for food use, the insect exoskeleton may offer the possibility for the production of added value products. Here, the aim was to isolate bacteria from the surface of farmed mealworms (Tenebrio molitor Linnaeus, 1758) for the production of chitinous material from insect exoskeletons using microbial fermentation. Isolates were screened for proteases and acid production that may aid deproteination and demineralisation of insects through fermentation to produce chitin. Selected isolates were used single-step (isolated bacteria only) or two-step fermentations with Lactobacillus plantarum (DSM 20174). Two-step fermentations with isolates from mealworm exoskeletons resulted in a demineralisation of 97.9 and 98.5% from deproteinated mealworm fractions. Attenuated total reflectance-Fourier- transform infrared spectroscopy analysis showed that crude chitin was produced. However, further optimisation is needed before the process can be upscaled. This is, to our knowledge, the first report using microbial fermentation for the extraction of chitin from insects. Bacterial isolates from farmed mealworms may aid deproteination and demineralisation of insects through fermentation to produce chitin.