Twin prime editor: seamless repair without damage
CRISPR-Cas9 creates remarkable possibilities to modify targeted regions in genomic DNA. However, CRISPR-Cas-mediated DNA double-stranded breaks (DSBs), that tend to generate random insertions or deletions, limit this technology. Recently, Anzalone et al. developed a 'twin prime editing' to...
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| Published in | Trends in biotechnology (Regular ed.) Vol. 40; no. 4; pp. 374 - 376 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
Elsevier Ltd
01.04.2022
Elsevier Limited |
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| Abstract | CRISPR-Cas9 creates remarkable possibilities to modify targeted regions in genomic DNA. However, CRISPR-Cas-mediated DNA double-stranded breaks (DSBs), that tend to generate random insertions or deletions, limit this technology. Recently, Anzalone et al. developed a 'twin prime editing' tool to replace, integrate, or delete large genomic DNA sequences without generating DNA DSBs. |
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| AbstractList | CRISPR-Cas9 creates remarkable possibilities to modify targeted regions in genomic DNA. However, CRISPR-Cas-mediated DNA double-stranded breaks (DSBs), that tend to generate random insertions or deletions, limit this technology. Recently, Anzalone et al. developed a 'twin prime editing' tool to replace, integrate, or delete large genomic DNA sequences without generating DNA DSBs. |
| Author | Amin, Imran Awan, Muhammad Jawad Akbar Mansoor, Shahid Ali, Zahir |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/35153078$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1038/s41587-021-00891-x 10.1038/s41587-021-00868-w 10.1038/s41477-019-0405-0 10.1038/s41586-019-1711-4 10.1038/s41467-021-21337-7 10.1186/s13059-020-02170-5 10.1038/s41587-020-0455-x |
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| DOI | 10.1016/j.tibtech.2022.01.013 |
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| Keywords | prime editor DNA deletion DSB-independent genome editing twin prime editing pegRNA DNA replacement |
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| References | Zhang (bb0045) 2019; 5 Hsu (bb0025) 2021; 12 Anzalone (bb0035) 2021 Anzalone (bb0005) 2019; 576 Jin (bb0015) 2021; 39 Lin (bb0020) 2021; 39 Jiang (bb0040) 2020; 21 Lin (bb0010) 2020; 38 Nelson (bb0030) 2021 Anzalone (10.1016/j.tibtech.2022.01.013_bb0005) 2019; 576 Jin (10.1016/j.tibtech.2022.01.013_bb0015) 2021; 39 Jiang (10.1016/j.tibtech.2022.01.013_bb0040) 2020; 21 Lin (10.1016/j.tibtech.2022.01.013_bb0010) 2020; 38 Zhang (10.1016/j.tibtech.2022.01.013_bb0045) 2019; 5 Lin (10.1016/j.tibtech.2022.01.013_bb0020) 2021; 39 Nelson (10.1016/j.tibtech.2022.01.013_bb0030) 2021 Hsu (10.1016/j.tibtech.2022.01.013_bb0025) 2021; 12 Anzalone (10.1016/j.tibtech.2022.01.013_bb0035) 2021 |
| References_xml | – volume: 39 start-page: 923 year: 2021 end-page: 927 ident: bb0020 article-title: High-efficiency prime editing with optimized, paired pegRNAs in plants publication-title: Nat. Biotechnol. contributor: fullname: Lin – volume: 39 start-page: 1292 year: 2021 end-page: 1299 ident: bb0015 article-title: Genome-wide specificity of prime editors in plants publication-title: Nat. Biotechnol. contributor: fullname: Jin – volume: 38 start-page: 582 year: 2020 end-page: 585 ident: bb0010 article-title: Prime genome editing in rice and wheat publication-title: Nat. Biotechnol. contributor: fullname: Lin – volume: 12 start-page: 1034 year: 2021 ident: bb0025 article-title: PrimeDesign software for rapid and simplified design of prime editing guide RNAs publication-title: Nat. Commun. contributor: fullname: Hsu – volume: 5 start-page: 480 year: 2019 end-page: 485 ident: bb0045 article-title: Generation of herbicide tolerance traits and a new selectable marker in wheat using base editing publication-title: Nat. Plants contributor: fullname: Zhang – year: 2021 ident: bb0030 article-title: Engineered pegRNAs improve prime editing efficiency publication-title: Nat. Biotechnol. contributor: fullname: Nelson – year: 2021 ident: bb0035 article-title: Programmable deletion, replacement, integration and inversion of large DNA sequences with twin prime editing publication-title: Nat. Biotechnol. contributor: fullname: Anzalone – volume: 21 start-page: 257 year: 2020 ident: bb0040 article-title: Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize publication-title: Genome Biol. contributor: fullname: Jiang – volume: 576 start-page: 149 year: 2019 end-page: 157 ident: bb0005 article-title: Search-and-replace genome editing without double-strand breaks or donor DNA publication-title: Nature contributor: fullname: Anzalone – volume: 39 start-page: 1292 year: 2021 ident: 10.1016/j.tibtech.2022.01.013_bb0015 article-title: Genome-wide specificity of prime editors in plants publication-title: Nat. Biotechnol. doi: 10.1038/s41587-021-00891-x contributor: fullname: Jin – year: 2021 ident: 10.1016/j.tibtech.2022.01.013_bb0035 article-title: Programmable deletion, replacement, integration and inversion of large DNA sequences with twin prime editing publication-title: Nat. Biotechnol. contributor: fullname: Anzalone – volume: 39 start-page: 923 year: 2021 ident: 10.1016/j.tibtech.2022.01.013_bb0020 article-title: High-efficiency prime editing with optimized, paired pegRNAs in plants publication-title: Nat. Biotechnol. doi: 10.1038/s41587-021-00868-w contributor: fullname: Lin – volume: 5 start-page: 480 year: 2019 ident: 10.1016/j.tibtech.2022.01.013_bb0045 article-title: Generation of herbicide tolerance traits and a new selectable marker in wheat using base editing publication-title: Nat. Plants doi: 10.1038/s41477-019-0405-0 contributor: fullname: Zhang – year: 2021 ident: 10.1016/j.tibtech.2022.01.013_bb0030 article-title: Engineered pegRNAs improve prime editing efficiency publication-title: Nat. Biotechnol. contributor: fullname: Nelson – volume: 576 start-page: 149 year: 2019 ident: 10.1016/j.tibtech.2022.01.013_bb0005 article-title: Search-and-replace genome editing without double-strand breaks or donor DNA publication-title: Nature doi: 10.1038/s41586-019-1711-4 contributor: fullname: Anzalone – volume: 12 start-page: 1034 year: 2021 ident: 10.1016/j.tibtech.2022.01.013_bb0025 article-title: PrimeDesign software for rapid and simplified design of prime editing guide RNAs publication-title: Nat. Commun. doi: 10.1038/s41467-021-21337-7 contributor: fullname: Hsu – volume: 21 start-page: 257 year: 2020 ident: 10.1016/j.tibtech.2022.01.013_bb0040 article-title: Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize publication-title: Genome Biol. doi: 10.1186/s13059-020-02170-5 contributor: fullname: Jiang – volume: 38 start-page: 582 year: 2020 ident: 10.1016/j.tibtech.2022.01.013_bb0010 article-title: Prime genome editing in rice and wheat publication-title: Nat. Biotechnol. doi: 10.1038/s41587-020-0455-x contributor: fullname: Lin |
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| SubjectTerms | Congenital diseases CRISPR CRISPR-Cas Systems Deoxyribonucleic acid DNA DNA Breaks, Double-Stranded DNA damage DNA deletion DNA Repair DNA replacement DSB-independent genome editing Efficiency Engineering Gene Editing Gene sequencing Genes Genomes Genomics Mutation Nucleotide sequence pegRNA prime editor Stem cells twin prime editing |
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| Title | Twin prime editor: seamless repair without damage |
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