Twin prime editor: seamless repair without damage

CRISPR-Cas9 creates remarkable possibilities to modify targeted regions in genomic DNA. However, CRISPR-Cas-mediated DNA double-stranded breaks (DSBs), that tend to generate random insertions or deletions, limit this technology. Recently, Anzalone et al. developed a 'twin prime editing' to...

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Bibliographic Details
Published inTrends in biotechnology (Regular ed.) Vol. 40; no. 4; pp. 374 - 376
Main Authors Awan, Muhammad Jawad Akbar, Ali, Zahir, Amin, Imran, Mansoor, Shahid
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.04.2022
Elsevier Limited
Subjects
Congenital diseases
CRISPR
CRISPR-Cas Systems
Deoxyribonucleic acid
DNA
DNA Breaks, Double-Stranded
DNA damage
DNA deletion
DNA Repair
DNA replacement
DSB-independent genome editing
Efficiency
Engineering
Gene Editing
Gene sequencing
Genes
Genomes
Genomics
Mutation
Nucleotide sequence
pegRNA
prime editor
Stem cells
twin prime editing
prime editor
DNA deletion
DSB-independent genome editing
twin prime editing
pegRNA
DNA replacement
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Summary:CRISPR-Cas9 creates remarkable possibilities to modify targeted regions in genomic DNA. However, CRISPR-Cas-mediated DNA double-stranded breaks (DSBs), that tend to generate random insertions or deletions, limit this technology. Recently, Anzalone et al. developed a 'twin prime editing' tool to replace, integrate, or delete large genomic DNA sequences without generating DNA DSBs.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0167-7799
1879-3096
DOI:10.1016/j.tibtech.2022.01.013