Ion-pair chromatography of acidic drug metabolites and endogenic compounds

Liquid-liquid chromatographic systems based on ion-pair partition with silica microparticles as the support for the stationary phase have been used for the separation of anionic compounds of biochemical and pharmacological interest. A high separating efficiency can be obtained with both aqueous and...

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Bibliographic Details
Published inJournal of chromatography Vol. 125; no. 1; p. 327
Main Authors Fransson, B, Wahlund, K G, Johansson, I M, Schill, G
Format Journal Article
LanguageEnglish
Published Netherlands 29.09.1976
Subjects
Barbiturates - analysis
Biogenic Amines - analysis
Carboxylic Acids - analysis
Chromatography, Ion Exchange
Glucuronates - analysis
Glycine - analysis
Hydrogen-Ion Concentration
Methods
Pharmaceutical Preparations
Phenols - analysis
Solvents
Steroids - analysis
Sulfonamides - analysis
Sulfuric Acids - analysis
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Summary:Liquid-liquid chromatographic systems based on ion-pair partition with silica microparticles as the support for the stationary phase have been used for the separation of anionic compounds of biochemical and pharmacological interest. A high separating efficiency can be obtained with both aqueous and organic mobile phases and the retention is easily regulated by the nature and the concentration of the quaternary ammonium counter ion, present in the aqueous phase. The influence of the composition of the liquid phases on the selectivity and separating efficiency has been studied, as well as equilibration methods and the stability of the systems. Examples are given of separations of sulphonamides, barbiturates, glucuronic and sulphuric acid conjugates of steroidal compounds and phenols glycine conjugates of carboxylic acids (hippuric, nicotinuric and salicyluric acid) and anionic metabolites of biogenic amines (indoleacetic, benzoic, mandelic and phenylacetic acid derivatives).
DOI:10.1016/s0021-9673(00)93829-9