Polymer: Bioceramic composites optimization by tetracycline addition

• Published in: International journal of pharmaceutics Vol. 336; no. 1; pp. 75 - 81
• Main Authors: Pataro, André L., Oliveira, Michele F., Teixeira, Karina I.R., Turchetti-Maia, Regina M.M., Lopes, Miriam T.P., Wykrota, Francisco H.L., Sinisterra, Rubén D., Cortés, Maria E.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 04.05.2007; Elsevier
• Subjects: Animals; Bioceramic; Biocompatibility; Biological and medical sciences; Bone Substitutes - chemistry; Bone Substitutes - metabolism; Bone Substitutes - pharmacology; Calcium Phosphates - chemistry; Cell Adhesion - drug effects; Cell Line; Cell Survival - drug effects; Ceramics - chemistry; Ceramics - pharmacology; Composite; Durapatite - chemistry; Fibroblasts - cytology; Fibroblasts - drug effects; General pharmacology; Implants, Experimental; Inflammation - pathology; Inflammation - prevention & control; Lactic Acid - chemistry; Lactic Acid - pharmacology; Male; Materials Testing; Medical sciences; Mice; Microscopy, Electron, Scanning; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Polyesters; Polyglycolic Acid - chemistry; Polyglycolic Acid - pharmacology; Polymer; Polymers - chemistry; Polymers - pharmacology; Porosity; Subcutaneous Tissue - drug effects; Subcutaneous Tissue - pathology; Surface Properties; Tetracycline; Tetracycline - chemistry; Tetracycline - pharmacology; Polymer; Biocompatibility; Composite; Bioceramic; Tetracycline; Pharmaceutical technology; Tetracycline derivatives; Protein synthesis inhibitor; Optimization
• ISSN: 0378-5173; 1873-3476
• DOI: 10.1016/j.ijpharm.2006.11.038

The aim of this study was to evaluate the biocompatibility of composites of poly-lactic acid polymer (PLA) and copolymer of lactic and glycolic acid (PLGA), dispersed in a bioceramic matrix, Osteosynt ® (BC), to which tetracycline (TC) was added. The in vitro test used direct contact test (ASTM F-813) and elution test (USP-XXIII, ISO 10993-5), and in vivo evaluation was performed after subcutaneous implantation in outbread Swiss mice. The 0.01% (w/w) TC addition did not affect composite cytotoxicity in vitro. The macroscopic and histologic evaluation in vivo after 1, 7, 13, 21, 28 and 56 days showed an initial intense infiltrate of inflammatory cells for most of the groups. The tissue showed normal pattern after 21 days for all the groups. TC addition exhibited significantly larger reduction of inflammation signs (Mann–Whitney test, p < 0.05) in the critical period of the resolution of the inflammatory process. Angiogenesis, cellular adsorption and fibrous deposit were observed on SEM evaluation. In conclusion, TC addition optimized composites polymer/bioceramic biocompatibility, contributing to anti-inflammatory response during the early phases of the wound healing process.