Purification of antifungal lipopeptides by reversed-phase high-performance liquid chromatography

• Published in: Journal of Chromatography A Vol. 639; no. 1; pp. 81 - 85
• Main Authors: Razafindralambo, Hary, Paquot, Michel, Hbid, Choukri, Jacques, Philippe, Destain, Jacqueline, Thonart, Philippe
• Format: Journal Article Web Resource
• Language: English
• Published: Netherlands Elsevier B.V 04.06.1993; Elsevier Science
• Subjects: Agriculture & agronomie; Agriculture & agronomy; Amino Acid Sequence; Amino Acids - analysis; Anti-Bacterial Agents; Antifungal Agents - chemistry; Antifungal Agents - isolation & purification; Antifungal Agents - pharmacology; antifungal properties; Bacillus subtilis; Bacillus subtilis - metabolism; Bacterial Proteins - chemistry; Bacterial Proteins - isolation & purification; Bacterial Proteins - pharmacology; Botrytis; Chromatography, Gel; Chromatography, Liquid - methods; Chromatography, Thin Layer; Fusarium; Life sciences; Lipopeptides; lipoproteins; Lipoproteins - chemistry; Lipoproteins - isolation & purification; Lipoproteins - pharmacology; Molecular Sequence Data; Peptides; Peptides, Cyclic - chemistry; Peptides, Cyclic - isolation & purification; Peptides, Cyclic - pharmacology; Sciences du vivant; Spectrophotometry, Ultraviolet
• ISSN: 0021-9673; 1873-3778
• DOI: 10.1016/0021-9673(93)83091-6

A rapid procedure for the purification of antifungal lipopeptides from Bacillus subtilis, a potential agent for biocontrol of plant diseases, was tested. It consists of a solid-phase extraction on C 18 gel followed by reversed-phase chromatography using a biocompatible PepRPC HR 5/5 column with a pharmacia fast protein liquid chromatographic system. This is a very effective method for isolating and fractionating iturin A and surfactin, two lipopeptides of different nature, co-produced by Bacillus subtilis strain S499. The presence of homologous lipopeptides was easily detected.