A fully automated flow-based approach for accelerated peptide synthesis

An automated method for solid-phase polypeptide synthesis capitalizes on rapid amide bond formation to enable the production of multiple traditionally difficult-to-synthesize sequences with both high yield and high purity. Here we report a fully automated, flow-based approach to solid-phase polypept...

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Bibliographic Details
Published inNature chemical biology Vol. 13; no. 5; pp. 464 - 466
Main Authors Mijalis, Alexander J, Thomas, Dale A, Simon, Mark D, Adamo, Andrea, Beaumont, Ryan, Jensen, Klavs F, Pentelute, Bradley L
Format Journal Article
LanguageEnglish
Published New York Nature Publishing Group US 01.05.2017
Nature Publishing Group
Subjects
631/92/611
631/92/96
639/638/549
Amino acids
Automation - methods
Biochemical Engineering
Biochemistry
Bioorganic Chemistry
brief-communication
Cell Biology
Chemical synthesis
Chemistry
Chemistry/Food Science
Peptides
Peptides - chemical synthesis
Peptides - chemistry
Phase transitions
Polypeptides
Solids
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Summary:An automated method for solid-phase polypeptide synthesis capitalizes on rapid amide bond formation to enable the production of multiple traditionally difficult-to-synthesize sequences with both high yield and high purity. Here we report a fully automated, flow-based approach to solid-phase polypeptide synthesis, with amide bond formation in 7 seconds and total synthesis times of 40 seconds per amino acid residue. Crude peptide purities and isolated yields were comparable to those for standard-batch solid-phase peptide synthesis. At full capacity, this approach can yield tens of thousands of individual 30-mer peptides per year.
ISSN:1552-4450
1552-4469
DOI:10.1038/nchembio.2318