A fully automated flow-based approach for accelerated peptide synthesis
An automated method for solid-phase polypeptide synthesis capitalizes on rapid amide bond formation to enable the production of multiple traditionally difficult-to-synthesize sequences with both high yield and high purity. Here we report a fully automated, flow-based approach to solid-phase polypept...
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Published in | Nature chemical biology Vol. 13; no. 5; pp. 464 - 466 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
New York
Nature Publishing Group US
01.05.2017
Nature Publishing Group |
Subjects | |
Online Access | Get full text |
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Summary: | An automated method for solid-phase polypeptide synthesis capitalizes on rapid amide bond formation to enable the production of multiple traditionally difficult-to-synthesize sequences with both high yield and high purity.
Here we report a fully automated, flow-based approach to solid-phase polypeptide synthesis, with amide bond formation in 7 seconds and total synthesis times of 40 seconds per amino acid residue. Crude peptide purities and isolated yields were comparable to those for standard-batch solid-phase peptide synthesis. At full capacity, this approach can yield tens of thousands of individual 30-mer peptides per year. |
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ISSN: | 1552-4450 1552-4469 |
DOI: | 10.1038/nchembio.2318 |