Three dimensional sea-urchin-like PdAuCu nanocrystals/ferrocene-grafted-polylysine as an efficient probe to amplify the electrochemical signals for ultrasensitive immunoassay of carcinoembryonic antigen

• Published in: Biosensors & bioelectronics Vol. 132; pp. 294 - 301
• Main Authors: Chen, Yao, Wang, Ai-Jun, Yuan, Pei-Xin, Luo, Xiliang, Xue, Yadong, Feng, Jiu-Ju
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01.05.2019
• Subjects: Antibodies, Immobilized - chemistry; Biosensing Techniques - methods; Carcinoembryonic antigen; Carcinoembryonic Antigen - blood; Copper - chemistry; Electrochemical immunosensor; Electrochemical Techniques - methods; Ferrocene-grafted-polylysine; Ferrous Compounds - chemistry; Gold - chemistry; Humans; Limit of Detection; Metal Nanoparticles - chemistry; Metal Nanoparticles - ultrastructure; Metallocenes - chemistry; Palladium - chemistry; Polylysine - chemistry; Sea-urchin-like nanocrystals; Carcinoembryonic antigen; Electrochemical immunosensor; Sea-urchin-like nanocrystals; Ferrocene-grafted-polylysine
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2019.02.057

A novel sandwich-like immunosensor was efficiently fabricated for detection of carcinoembryonic antigen (CEA) with three dimensional sea-urchin-like PdAuCu nanocrystals (PdAuCu NCs)/ferrocene-grafted-polylysine (Fc-g-PLL) as the label of secondary antibodies (Ab2) and Au nanoparticles (Au NPs) as the substrate material. Herein, PdAuCu NCs were directly synthesized with polyethylene oxide (PEO) as a growth-directing agent by a facile one-step aqueous method without any organic solvent. Meanwhile, Fc-g-PLL was obtained by covalent linkage of Fc with PLL via Schiff-base reaction. The well-dispersed PdAuCu NCs by Fc-g-PLL have the enlarged surface area, enhanced catalytic properties and superior biocompatibility to amplify the current signals. The resultant immunosensor shows linear relationship of the electrochemical responses with the CEA concentrations within a broader linear range (0.001–100.0 ng mL–1) and a lower detection limit (0.23 pg mL–1, S/N = 3). Furthermore, the immunosensor was explored for practical assay of CEA in human serum samples with accredited results. The novel immunoassay provides a feasible platform for early medical diagnosis. [Display omitted] •3D sea-urchin-like PdAuCu NCs were synthesized by a one-step aqueous method.•Fc-g-PLL was firstly prepared as an electroactive probe.•PdAuCu NCs/Fc-g-PLL acted as an Ab2 label to amplify the electrochemical signals.•The immunosensor for CEA displayed a broader linear range and a lower detection limit.