Transcriptome Analysis of Populus euphratica under Salt Treatment and PeERF1 Gene Enhances Salt Tolerance in Transgenic Populus alba × Populus glandulosa

• Published in: International journal of molecular sciences Vol. 23; no. 7; p. 3727
• Main Authors: Ge, Xiao-Lan, Zhang, Lei, Du, Jiu-Jun, Wen, Shuang-Shuang, Qu, Guan-Zheng, Hu, Jian-Jun
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28.03.2022; MDPI
• Subjects: Abiotic stress; Antioxidants; Desert environments; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant; Genes; Genetic engineering; Genetically modified organisms; Hot climates; Metabolism; Oxidative stress; PeERF1; Physiology; Plant Breeding; Plants, Genetically Modified - genetics; Poplar; Populus - metabolism; Populus alba; Populus euphratica; Populus glandulosa; Resistance factors; Salinity tolerance; Salt; salt stress; Salt Tolerance - genetics; Stress response; Stress, Physiological - genetics; transcriptome; Transcriptomes; Transgenic plants; WGCNA; China; Populus euphratica; salt stress; WGCNA; transcriptome; PeERF1
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms23073727

is mainly distributed in desert environments with dry and hot climate in summer and cold in winter. Compared with other poplars, is more resistant to salt stress. It is critical to investigate the transcriptome and molecular basis of salt tolerance in order to uncover stress-related genes. In this study, salt-tolerant treatment of resulted in an increase in osmo-regulatory substances and recovery of antioxidant enzymes. To improve the mining efficiency of candidate genes, the analysis combining both the transcriptome WGCNA and the former GWAS results was selected, and a range of key regulatory factors with salt resistance were found. The gene was highly connected in the turquoise modules with significant differences in salt stress traits, and the expression levels were significantly different in each treatment. For further functional verification of , we obtained stable overexpression and dominant suppression transgenic lines by transforming into . The growth and physiological characteristics of the overexpressed plants were better than that of the wild type under salt stress. Transcriptome analysis of leaves of transgenic lines and WT revealed that highly enriched GO terms in DEGs were associated with stress responses, including abiotic stimuli responses, chemical responses, and oxidative stress responses. The result is helpful for in-depth analysis of the salt tolerance mechanism of poplar. This work provides important genes for poplar breeding with salt tolerance.