Evaluation of systemic immunity in atypical cutaneous leishmaniasis caused by Leishmania (L.) infantum chagasi

• Published in: Parasite immunology Vol. 44; no. 1-2; pp. e12896 - n/a
• Main Authors: Laurenti, Márcia Dalastra, Sosa‐Ochoa, Wilfredo, Araujo Flores, Gabriela Venicia, Sandoval Pacheco, Carmen Maria, Tomokane, Thaise Yumie, Oliveira, Luanda Mara da Silva, Zúniga, Concepción, Silveira, Fernando Tobias, Corbett, Carlos Eduardo Pereira
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.01.2022
• Subjects: Antigens; Cell-mediated immunity; cellular immune response; Cutaneous leishmaniasis; Cytokines; Enzyme-linked immunosorbent assay; Helper cells; Humans; humoral immune response; Humoral immunity; Immune response (humoral); Immunity, Cellular; Immunoglobulin G; Immunoglobulin M; Immunoglobulins; Interferon; Leishmania; Leishmania infantum; Leishmania infantum chagasi; Leishmaniasis; Leishmaniasis, Cutaneous; Leishmaniasis, Visceral - diagnosis; Lymphocytes T; Parasitic diseases; Patients; Tumor necrosis factor; Visceral leishmaniasis; cellular immune response; Leishmaniasis; humoral immune response; Leishmania infantum chagasi
• ISSN: 0141-9838; 1365-3024
• DOI: 10.1111/pim.12896

In some central‐American countries, Leishmania (L.) infantum chagasi infection can cause non‐ulcerated or atypical cutaneous leishmaniasis (NUCL) in addition to the classic clinical form, visceral leishmaniasis (VL). Little is known about the host‐parasite relationship that can contribute to the determination of one or another clinical form. The present study had the objective to evaluate the humoral and cellular immunity in the sera of individuals affected by NUCL to improve the comprehension of this atypical host‐parasite interaction. Based on clinical and laboratory diagnosis, serum of 80 individuals was collected to evaluate the cytokines and immunoglobulins profile of NUCL (n = 47), VL patients (n = 5), and negative controls (n = 28). Cytokines were detected using Cytokine Bead Array (CBA) Human Th1/Th2/Th17 kit according to the manufacturer's instructions; class (IgG and IgM), and subclass of (IgG1 and IgG2) immunoglobulins was evaluated by ELISA using specific antigens. The concentration of TNF‐α, IFN‐γ, IL‐2 and IL‐4 cytokines in NUCL, VL and control was present below the detection threshold of CBA kit. IL‐6, IL‐10 and IL‐17A cytokines was lower in NUCL compared to LV patients. Regarding to immunoglobulins, NUCL patients produced 4.0 times more IgG than the control, while VL patients produced 6.6 times more; and IgM level was 1.6 times higher in NUCL and 2.6 times in VL patients compared to the control. Concerning the immunoglobulins subclass, only VL patients showed positive reaction for IgG1, and IgG2 did not show positive reaction among the groups. The results showed a weak cellular and humoral systemic immune response in NUCL patients.