Metabolic Engineering and Regulation of Diol Biosynthesis from Renewable Biomass in Escherichia coli

• Published in: Biomolecules (Basel, Switzerland) Vol. 12; no. 5; p. 715
• Main Authors: Wu, Tong, Liu, Yumei, Liu, Jinsheng, Chen, Zhenya, Huo, Yi-Xin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 18.05.2022; MDPI
• Subjects: Alcohols; Amino acids; Biofuels; Biomass; Biosynthesis; Carbon; Carbon - metabolism; Carbon sources; Catalysis; Cosmetics; Dehydrogenases; diols; E coli; Energy resources; Enzymes; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Genetic engineering; Glucose; Kinases; Metabolic Engineering; Metabolic networks; Metabolism; Microorganisms; Optimization; Productivity; regulation; Review; carbon sources; diols; metabolic engineering; regulation; Escherichia coli
• ISSN: 2218-273X; 2218-273X
• DOI: 10.3390/biom12050715

As bulk chemicals, diols have wide applications in many fields, such as clothing, biofuels, food, surfactant and cosmetics. The traditional chemical synthesis of diols consumes numerous non-renewable energy resources and leads to environmental pollution. Green biosynthesis has emerged as an alternative method to produce diols. as an ideal microbial factory has been engineered to biosynthesize diols from carbon sources. Here, we comprehensively summarized the biosynthetic pathways of diols from renewable biomass in and discussed the metabolic-engineering strategies that could enhance the production of diols, including the optimization of biosynthetic pathways, improvement of cofactor supplementation, and reprogramming of the metabolic network. We then investigated the dynamic regulation by multiple control modules to balance the growth and production, so as to direct carbon sources for diol production. Finally, we proposed the challenges in the diol-biosynthesis process and suggested some potential methods to improve the diol-producing ability of the host.