Plant genomic DNA isolation: an art or a science

Isolating quality DNA from tissues/cells presents a variety of problems in particular when plants are used as the source material. The specific characteristics of plants like the presence of rigid polysaccharide cell wall, pigments, chemical heterogeneity of secondary metabolites found in diverse sp...

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Bibliographic Details
Published inBiotechnology journal Vol. 2; no. 3; p. 386
Main Authors Varma, Astha, Padh, Harish, Shrivastava, Neeta
Format Journal Article
LanguageEnglish
Published Germany 01.03.2007
Subjects
Biotechnology - methods
DNA, Plant - chemistry
DNA, Plant - isolation & purification
Flavonoids - chemistry
Genome, Plant
Phenols - chemistry
Plants - genetics
Polyphenols
Polysaccharides - chemistry
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Summary:Isolating quality DNA from tissues/cells presents a variety of problems in particular when plants are used as the source material. The specific characteristics of plants like the presence of rigid polysaccharide cell wall, pigments, chemical heterogeneity of secondary metabolites found in diverse species of plants, etc., necessitate special consideration and skill during isolation procedure. Until now, numerous protocols have been published for the purpose, but none is found to be universally applicable. Various factors starting from the selection of source material to the concentration of metabolites present in the plant decide the course of the isolation procedure. The present review is an update of various methods used for plant genomic DNA isolation, and it epitomizes the various problems faced and the solutions made to contend with them during DNA isolation from plant cells.
ISSN:1860-7314
DOI:10.1002/biot.200600195