Leishmaniasis in cat shelters: A serological, molecular and entomological study

An epidemiological Leishmania spp. and entomological Phlebotomine sandflies survey was performed in cat shelters at leishmaniasis endemic area of Brazil. Blood and conjunctival swab (CS) samples were collected from 94 cats in two animal protection shelters. These samples were subjected to serologica...

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Published inTransboundary and emerging diseases Vol. 67; no. 5; pp. 2013 - 2019
Main Authors Leonel, João Augusto Franco, Vioti, Geovanna, Alves, Maria Luana, Benassi, Julia Cristina, Silva, Diogo Tiago da, Spada, Julio Cesar Pereira, Ruiz, Vera Letticie de Azevedo, Starke-Buzetti, Wilma Aparecida, Soares, Rodrigo Martins, Oliveira, Trícia Maria Ferreira de Sousa
Format Journal Article
LanguageEnglish
Published Germany Hindawi Limited 01.09.2020
Subjects
Addition polymerization
Antibodies
Cats
Deoxyribonucleic acid
DNA
Enzyme-linked immunosorbent assay
Epidemiology
Felis catus
Immunofluorescence
Leishmania
Leishmania spp
Leishmaniasis
Lutzomyia
Lutzomyia longipalpis
Parasitic diseases
PCR
Polls & surveys
Polymerase chain reaction
Serological tests
Serology
Shelters
Vector-borne diseases
Felis catus
Lutzomyia longipalpis
PCR
serology
Leishmania spp
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Summary:An epidemiological Leishmania spp. and entomological Phlebotomine sandflies survey was performed in cat shelters at leishmaniasis endemic area of Brazil. Blood and conjunctival swab (CS) samples were collected from 94 cats in two animal protection shelters. These samples were subjected to serological tests using the indirect immunofluorescence antibody test (IFAT) and indirect enzyme‐linked immunosorbent assay (ELISA) and to molecular test by polymerase chain reaction (PCR). In addition, a Phlebotomine sandflies survey was performed in the same shelters. The analyses revealed a positivity of 31.91% (30/94) through ELISA and 29.79% (28/94) through IFAT. The two serological tests showed a positive association with perfect agreement (k = 0.925). None of the cats were positive by Leishmania spp. DNA. One Lutzomyia (Lutzomyia) longipalpis male was found in one of the cat shelters. The results and the implications of our findings are discussed below.
Bibliography:Funding information
This research received financial support provided by São Paulo Research Foundation (FAPESP) under project number 2014/02882‐3.
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ISSN:1865-1674
1865-1682
DOI:10.1111/tbed.13544