Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis

• Published in: International journal of molecular sciences Vol. 23; no. 10; p. 5353
• Main Authors: Ramírez, Daniel, Rodríguez, María Esther, Cross, Ismael, Arias-Pérez, Alberto, Merlo, Manuel Alejandro, Anaya, Marco, Portela-Bens, Silvia, Martínez, Paulino, Robles, Francisca, Ruiz-Rejón, Carmelo, Rebordinos, Laureana
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11.05.2022; MDPI
• Subjects: Abundance; Artificial chromosomes; Biological evolution; Chromatin remodeling; Chromosome 11; Chromosome 16; chromosome evolution; Chromosomes; Cloning; cytogenomics; Deoxyribonucleic acid; DNA; Evolution; Fish; Gene mapping; Genes; genetic maps; Genomes; Genomics; Integration; karyotype; Karyotypes; Microsatellites; Morphology; Nucleotide sequence; pleuronectiformes; Solea senegalensis; Synteny; Transposons; Vertebrates; genetic maps; karyotype; chromosome evolution; repetitive sequences; Solea senegalensis; cytogenomics; comparative genomics; pleuronectiformes
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms23105353

The Pleuronectiformes order, which includes several commercially-important species, has undergone extensive chromosome evolution. One of these species is , a flatfish with 2 = 42 chromosomes. In this study, a cytogenomics approach and integration with previous maps was applied to characterize the karyotype of the species. Synteny analysis of was carried out using two flatfish as a reference: and . Most chromosomes (or chromosome arms for metacentrics and submetacentrics) showed a one-to-one macrosyntenic pattern with the other two species. In addition, we studied how repetitive sequences could have played a role in the evolution of bi-armed (3, and 5-9) and acrocentric (11, 12 and 16) chromosomes, which showed the highest rearrangements compared with the reference species. A higher abundance of TEs (Transposable Elements) and other repeated elements was observed adjacent to telomeric regions on chromosomes 3, 7, 9 and 16. However, on chromosome 11, a greater abundance of DNA transposons was detected in interstitial BACs. This chromosome is syntenic with several chromosomes of the other two flatfish species, suggesting rearrangements during its evolution. A similar situation was also found on chromosome 16 (for microsatellites and low complexity sequences), but not for TEs (retroelements and DNA transposons). These differences in the distribution and abundance of repetitive elements in chromosomes that have undergone remodeling processes during the course of evolution also suggest a possible role for simple repeat sequences in rearranged regions.