Human mesenchymal factors induce rat hippocampal‐ and human neural stem cell dependent oligodendrogenesis
The generation of new oligodendrocytes is essential for adult brain repair in diseases such as multiple sclerosis. We previously identified the multifunctional p57kip2 protein as a negative regulator of myelinating glial cell differentiation and as an intrinsic switch of glial fate decision in adult...
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Published in | Glia Vol. 66; no. 1; pp. 145 - 160 |
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Main Authors | , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Wiley Subscription Services, Inc
01.01.2018
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Subjects | |
Online Access | Get full text |
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Summary: | The generation of new oligodendrocytes is essential for adult brain repair in diseases such as multiple sclerosis. We previously identified the multifunctional p57kip2 protein as a negative regulator of myelinating glial cell differentiation and as an intrinsic switch of glial fate decision in adult neural stem cells (aNSCs). In oligodendroglial precursor cells (OPCs), p57kip2 protein nuclear exclusion was recently found to be rate limiting for differentiation to proceed. Furthermore, stimulation with mesenchymal stem cell (MSC)‐derived factors enhanced oligodendrogenesis by yet unknown mechanisms. To elucidate this instructive interaction, we investigated to what degree MSC secreted factors are species dependent, whether hippocampal aNSCs respond equally well to such stimuli, whether apart from oligodendroglial differentiation also tissue integration and axonal wrapping can be promoted and whether the oligodendrogenic effect involved subcellular translocation of p57kip2. We found that CC1 positive oligodendrocytes within the hilus express nuclear p57kip2 protein and that MSC dependent stimulation of cultured hippocampal aNSCs was not accompanied by nuclear p57kip2 exclusion as observed for parenchymal OPCs after spontaneous differentiation. Stimulation with human MSC factors was observed to equally promote rat stem cell oligodendrogenesis, axonal wrapping and tissue integration. As forced nuclear shuttling of p57kip2 led to decreased CNPase‐ but elevated GFAP expression levels, this indicates heterogenic oligodendroglial mechanisms occurring between OPCs and aNSCs. We also show for the first time that dominant pro‐oligodendroglial factors derived from human fetal MSCs can instruct human induced pluripotent stem cell‐derived NSCs to differentiate into O4 positive oligodendrocytes.
Main Points
Human fetal MSCs potently instruct oligodendrogenesis from adult rat hippocampal‐ and hIPSC‐ derived neural stem cells.
Oligodendrocyte generation does not depend on nuclear p57kip2 protein shuttling as opposed to parenchymal OPC differentiation. |
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Bibliography: | Funding information This work was supported by the German Research Foundation (DFG; SPP1757/KU1934/2_1, KU1934/5‐1), the Christiane and Claudia Hempel Foundation for clinical stem cell research, DMSG Ortsvereinigung Düsseldorf und Umgebung e.V., iBrain, YoungGlia, the German Academic Exchange Service (DAAD) and FONDECYT‐CONICYT (Chile; regular grant number 1161787). A.P. is supported by a young investigator grant from the Bundesministerium für Bildung und Forschung (BMBF e:Bio). The MS Center at the Department of Neurology is supported in part by the Walter and Ilse Rose Foundation and the James and Elisabeth Cloppenburg, Peek & Cloppenburg Düsseldorf Foundation. R. O. is supported by grants from the BBSRC (LO21072/1) and MRC (MR/K026682/1). J.A acknowledges funding from the medical faculty of Heinrich Heine University, Düsseldorf. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0894-1491 1098-1136 |
DOI: | 10.1002/glia.23233 |