miR 31-3p Has the Highest Expression in Cesarean Scar Endometriosis

Micro-RNAs expression can vary between different forms of endometriosis, but data on miRNA expression in cesarean scar endometriosis is lacking. The present study is comprised of 30 patients with endometriosis in the cesarean scar (scar endometriosis, SE), 14 patients with deep infiltrating endometr...

Full description

Saved in:
Bibliographic Details
Published inInternational journal of molecular sciences Vol. 23; no. 9; p. 4660
Main Authors Szubert, Maria, Nowak-Glück, Anna, Domańska-Senderowska, Daria, Szymańska, Bożena, Sowa, Piotr, Rycerz, Aleksander, Wilczyński, Jacek R
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 22.04.2022
MDPI
Subjects
Cell cycle
cesarean scar endometriosis
deep infiltrating endometriosis
endometrial cyst
Endometriosis
Endometrium
Gene expression
Mesothelioma
MicroRNAs
miRNA
Ovarian cancer
Paraffins
Thyroid cancer
endometriosis
cesarean scar endometriosis
miRNA
deep infiltrating endometriosis
endometrial cyst
Online AccessGet full text

Cover

More Information
Summary:Micro-RNAs expression can vary between different forms of endometriosis, but data on miRNA expression in cesarean scar endometriosis is lacking. The present study is comprised of 30 patients with endometriosis in the cesarean scar (scar endometriosis, SE), 14 patients with deep infiltrating endometriosis (DIE), 47 patients with endometrioma (ovarian endometrial cyst, OE), and 33 patients with healthy ovarian tissue as the control group (CG). In the initial experiment to identify possible dysregulated miRNAs, the levels of 754 miRNAs in formalin-fixed paraffin-embedded tissue (FFPE) samples from OE, high-grade ovarian cancer, endometrioid ovarian cancer, and CG were measured. We identified seven potentially dysregulated miRNAs: miR-1-3p, miR-31-3p, miR-125b-1-3p, miR-200b-3p, miR-548d, miR-502, and miR-503. We then examined the expression profiles of each of these miRNAs individually in the SE, DIE, OE, and CG FFPE samples using RT-qPCR. miR-31-3p had significantly higher levels of expression and miR-125b-1-3p had significantly lower levels of expression in SE compared to the controls. Overall, the higher expression levels of miR-31-3p and the lower expression levels of miR-125b-1-3p are consistent with the benign nature of SE. Importantly, the results of the present study demonstrate the possibility of using miRNA to monitor the risk of malignant transformation of endometriosis tissue.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23094660