Fungal secondary metabolism is governed by an RNA-binding protein CsdA/RsdA complex

• Published in: Nature communications Vol. 14; no. 1; p. 7351
• Main Authors: Song, Zili, Zhou, Shuang, Zhang, Hongjiao, Keller, Nancy P, Oakley, Berl R, Liu, Xiao, Yin, Wen-Bing
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 14.11.2023; Nature Portfolio
• Subjects: Epigenetics; Fungi; Gene Expression Profiling; Metabolism; Metabolites; Metabolomics; Post-transcription; Protein turnover; Proteins; Ribonucleic acid; RNA; RNA Precursors; RNA-binding protein; RNA-Binding Proteins - genetics; Secondary Metabolism - genetics; Secondary metabolites; Transcriptomes
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-023-43205-2

Production of secondary metabolites is controlled by a complicated regulatory network in eukaryotic cells. Several layers of regulators are involved in this process, ranging from pathway-specific regulation, to epigenetic control, to global regulation. Here, we discover that interaction of an RNA-binding protein CsdA with a regulator RsdA coordinates fungal secondary metabolism. Employing a genetic deletion approach and transcriptome analysis as well as metabolomics analysis, we reveal that CsdA and RsdA synergistically regulate fungal secondary metabolism comprehensively. Mechanistically, comprehensive genetic and biochemical studies prove that RsdA and CsdA co-localize in the nucleus and physically interact to achieve their functions. In particular, we demonstrate that CsdA mediates rsdA expression by binding specific motif "GUCGGUAU" of its pre-mRNA at a post-transcriptional level. We thus uncover a mechanism in which RNA-binding protein physically interacts with, and controls the expression level of, the RsdA to coordinate fungal secondary metabolism.