Oxidative metabolism of 7-methoxy-2-nitro-naphtho[2,1-b]furan (R 7000) by the microsomal system isolated from 3-methylcholanthrene-induced rat liver

The metabolism of 7-methoxy-2-nitro-naphtho[2,1-b]furan and the subsequent binding to DNA, under aerobic conditions, were investigated using liver microsomes of both untreated rats and rats pre-treated with 3-methylcholanthrene [3-MC]. The metabolites were analyzed by HPLC. The following compounds:...

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Published inCarcinogenesis (New York) Vol. 9; no. 11; p. 1987
Main Authors Strapélias, H, Lhoste, J M, Royer, R, Buisson, J P, Duquesne, M
Format Journal Article
LanguageEnglish
Published England 01.11.1988
Subjects
Animals
Chromatography, High Pressure Liquid
Enzyme Induction - drug effects
In Vitro Techniques
Magnetic Resonance Spectroscopy
Male
Mass Spectrometry
Methylcholanthrene - pharmacology
Microsomes, Liver - metabolism
Nitrofurans - metabolism
Phenobarbital - pharmacology
Rats
Rats, Inbred Strains
Solubility
Spectrum Analysis
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Summary:The metabolism of 7-methoxy-2-nitro-naphtho[2,1-b]furan and the subsequent binding to DNA, under aerobic conditions, were investigated using liver microsomes of both untreated rats and rats pre-treated with 3-methylcholanthrene [3-MC]. The metabolites were analyzed by HPLC. The following compounds: 7-hydroxy-2-nitro-naphtho[2,1-b]-furan-6,9-dione; 6,7-dihydro-2-nitro-naphtho[2,1-b]furan; 7-hydroxy-2-nitro-naphtho[2,1-b]furan and 6-hydroxy-7-methoxy-2-nitro-naphtho[2,1-b]furan have been identified by their UV-visible, mass spectra, NMR spectra and by comparison to an authentic reference sample. Qualitative and quantitative metabolic charts involving only ring oxidation have been established.
ISSN:0143-3334
DOI:10.1093/carcin/9.11.1987