Differential expression of Prx I and II in mouse testis and their up-regulation by radiation

• Published in: Biochemical and biophysical research communications Vol. 296; no. 2; pp. 337 - 342
• Main Authors: Lee, Keesook, Park, Ji-Sun, Kim, Yun-Jeong, Soo Lee, Yong, Sook Hwang, Tae, Kim, Dae-Joong, Park, Eun-Mi, Park, Young-Mee
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 16.08.2002
• Subjects: Animals; Antioxidants - metabolism; Apoptosis - physiology; Gene Expression Regulation, Developmental; Glutathione - metabolism; In Situ Nick-End Labeling; Leydig and Sertoli cells; Lipid Peroxidation; Male; Mice; Mice, Inbred C57BL; Oxidative stress; Peroxidases - genetics; Peroxidases - metabolism; Peroxiredoxin; Peroxiredoxins; Radiation; Testis; Testis - cytology; Testis - growth & development; Testis - metabolism; Testis - radiation effects; Thiobarbituric Acid Reactive Substances - metabolism; Up-Regulation - radiation effects; X-Rays; Peroxiredoxin; Testis; Oxidative stress; Leydig and Sertoli cells; Radiation
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/S0006-291X(02)00801-X

Testis is one of the most sensitive organs to ionizing radiation. The present study was designed to unravel the possible role of antioxidant proteins, peroxiredoxin I and II (Prx I and II) in the testis. Our results show that Prx I and II are constitutively expressed in the testis and their expression levels are decreased to some extent as the testis develops. Interestingly, immunohistochemical analysis revealed a preferential expression of Prx I and II in Leydig and Sertoli cells, respectively. Neither Prx I nor Prx II expression was obvious in the testicular germ cells including spermatogonia and spermatocytes. Ionizing radiation exerted oxidative stress on the testis and induced apoptosis primarily in the germ cells. When the irradiated testis was examined, the Prx system was found to be transiently up-regulated. Taken together, we suggest that the relative radiation-resistance of Leydig and Sertoli cells could be attributed in part to the antioxidant function of the Prx system in these cells.