Isolation and identification of two new compounds from marine-derived fungus Acremonium fusidioides RZ01

• Published in: Chinese journal of natural medicines Vol. 14; no. 12; pp. 934 - 938
• Main Authors: AN, Xiao, FENG, Bao-Min, CHEN, Gang, CHEN, Shao-Fei, WANG, Hai-Feng, PEI, Yue-Hu
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.12.2016
• Subjects: Acremonium - chemistry; Acremonium - classification; Acremonium - isolation & purification; Acremonium - metabolism; Acremonium fusidioides; Antineoplastic Agents - chemistry; Antineoplastic Agents - isolation & purification; Antineoplastic Agents - pharmacology; Cell Proliferation - drug effects; Chemical constituent; Fermentation; Fusidione; HL-60 Cells; Humans; Magnetic Resonance Spectroscopy; Molecular Structure; Seawater - microbiology; Steroid; Steroid; Acremonium fusidioides; Chemical constituent; Fusidione
• ISSN: 1875-5364; 1875-5364
• DOI: 10.1016/S1875-5364(17)30019-5

Two new compounds, (22E)-25-carboxy-8β,14β-epoxy-4α,5α-dihydroxyergosta-2,22-dien-7-one (1) and fusidione (3), along with two known compounds, 5α,8α-epidioxy ergosta-6,22-diene-3β-ol (2) and microperfuranone (4), were isolated from the fermentation products of the marine-sourced fungus Acremonium fusidioides RZ01. The structures of compounds 1 and 3 were elucidated by extensive spectroscopic methods, especially 2D NMR, and their absolute configurations were suggested on the basis of the circular dichroism spectral analysis and the NOESY data. Both new compounds showed inhibitory activity against HL-60 cells with IC50 values being16.6 and 44.9 μmol·L−1, respectively.