Diversity of bacterial endophytes in roots of Mexican husk tomato plants (Physalis ixocarpa) and their detection in the rhizosphere

• Published in: Genetics and molecular research Vol. 9; no. 4; pp. 2372 - 2380
• Main Authors: Marquez-Santacruz, H A, Hernandez-Leon, R, Orozco-Mosqueda, M C, Velazquez-Sepulveda, I, Santoyo, G
• Format: Journal Article
• Language: English
• Published: Brazil 01.01.2010
• Subjects: Actinobacteria; Bacilli; Bacillus; Bacteria - classification; Bacteria - genetics; Base Sequence; Burkholderia; DNA Primers; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; Endophytes; Homology; Lycopersicon esculentum; Lycopersicon esculentum - genetics; Lycopersicon esculentum - microbiology; Microbacterium; Plant Roots - genetics; Plant Roots - microbiology; Polymerase Chain Reaction; Pseudomonas; Rhizosphere; Roots; rRNA 16S; Soil microorganisms; Stenotrophomonas
• ISSN: 1676-5680; 1676-5680
• DOI: 10.4238/vol9-4gmr921

Endophytic bacterial diversity was estimated in Mexican husk tomato plant roots by amplified rDNA restriction analysis and sequence homology comparison of the 16S rDNA genes. Sixteen operational taxonomic units from the 16S rDNA root library were identified based on sequence analysis, including the classes Gammaproteobacteria, Betaproteobacteria, Actinobacteria, and Bacilli. The predominant genera were Stenotrophomonas (21.9%), Microbacterium (17.1%), Burkholderia (14.3%), Bacillus (14.3%), and Pseudomonas (10.5%). In a 16S rDNA gene library of the same plant species' rhizosphere, only common soil bacteria, including Stenotrophomonas, Burkholderia, Bacillus, and Pseudomonas, were detected. We suggest that the endophytic bacterial diversity within the roots of Mexican husk tomato plants is a subset of the rhizosphere bacterial population, dominated by a few genera.