A comparative study of protein patterns of human estrogen receptor positive (MCF-7) and negative (MDA-MB-231) breast cancer cell lines

• Published in: General physiology and biophysics Vol. 35; no. 3; p. 387
• Main Authors: Flodrova, Dana, Toporova, Lucia, Macejova, Dana, Lastovickova, Marketa, Brtko, Julius, Bobalova, Janette
• Format: Journal Article
• Language: English
• Published: Slovakia 01.07.2016
• Subjects: Biomarkers, Tumor - metabolism; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Gene Expression Regulation, Neoplastic; Humans; MCF-7 Cells; Neoplasm Proteins - metabolism; Proteome - metabolism; Receptors, Estrogen - metabolism
• ISSN: 0231-5882
• DOI: 10.4149/gpb_2016009

In the present study, we analyzed the cell lysates of human tumour cell lines representing two major clinically different types of breast cancer. Our main goal was to show the differences between them on proteomic level. Gel electrophoresis followed by MALDI-TOF MS analysis was used for proteins determination. Exactly 98 proteins were unequivocally identified and 60 of them were expressed differentially between MDA-MB-231 and MCF-7 cell lines. Among the proteins reported here, some well-known breast cancer markers (e.g., annexin A1, annexin A2 and vimentin) were identified in the MDA-MB-231 cell line and thus we were able to distinguish both cell lines sufficiently.