Study of the Complex between RNA and DEAE-Dextran

DEAE-dextran is an important substance used for transfer of nucleic acids into cultured mammalian cells. The transfection of a mammalian cell is accomplished via the complexation between nucleic acids and DEAE-dextran. Such complexes were thus synthesized using the RNA from a yeast under various con...

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Bibliographic Details
Published inKOBUNSHI RONBUNSHU Vol. 61; no. 2; pp. 139 - 143
Main Authors ONISHI, Yasuhiko, KIKUCHI, Yasuo
Format Journal Article
LanguageJapanese
Published The Society of Polymer Science, Japan 01.01.2004
Subjects
Complex
DEAE-dextran
DSC Analysis
IR Absorption
pH
RNA
Single DEAE-group
Tandem DEAE-group
Transfection
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Summary:DEAE-dextran is an important substance used for transfer of nucleic acids into cultured mammalian cells. The transfection of a mammalian cell is accomplished via the complexation between nucleic acids and DEAE-dextran. Such complexes were thus synthesized using the RNA from a yeast under various conditions including reaction time and pH, and analyzed by IR absorption spectroscopy and DSC. From the relation between the yield, pH, and reaction time, the optimum pH was in the range 5-10. The relation between the weight ratio of RNA/DEAE-dextran in the complex, pH, and reaction time indicated the ratio to be 0.25-0.55 in the pH range 5-10. The IR absorption spectra and DSC curves confirmed that the complexes were formed by the coulomb force between a single and tandem DEAE-group in DEAE-dextran and phosphoric acid in the RNA.
ISSN:0386-2186
1881-5685
DOI:10.1295/koron.61.139