Molecular cloning and expression analysis of two sex-lethal homolog genes during development in the oriental river prawn, Macrobrachium nipponense

• Published in: Genetics and molecular research Vol. 12; no. 4; pp. 4698 - 4711
• Main Authors: Zhang, Y P, Qiao, H, Zhang, W Y, Sun, S M, Jiang, S F, Gong, Y S, Xiong, Y W, Jin, S B, Fu, H T
• Format: Journal Article
• Language: English
• Published: Brazil 18.10.2013
• Subjects: Amino Acid Sequence; Animals; Arthropod Proteins - genetics; Base Sequence; Cloning, Molecular; Female; Gene Expression Regulation, Developmental; Larva - genetics; Larva - metabolism; Male; Metamorphosis, Biological; Molecular Sequence Data; Organ Specificity; Palaemonidae - genetics; Palaemonidae - growth & development; Palaemonidae - metabolism; Phylogeny; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA-Binding Proteins - genetics; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Sex Determination Processes; Transcriptome
• ISSN: 1676-5680; 1676-5680
• DOI: 10.4238/2013.October.18.8

In this study, two Sxl gene homologs, designated as Mnsxl1 and Mnsxl2, were cloned and characterized from the freshwater prawn Macrobrachium nipponense by rapid amplification of cDNA ends. The deduced amino acid sequences of Mnsxl1 and Mnsxl2 showed high sequence homology to the insect Sxl and contained conserved domains in two RNA-binding motifs. Real-time quantitative reverse transcription-polymerase chain reaction (RT-QPCR) showed that the Mnsxl1 and Mnsxl2 genes were expressed in all investigated tissues, with the highest level of expression in the intestine and liver. RT-QPCR also revealed that Mnsxl1 and Mnsxl2 mRNAs expressions were both significantly increased at 5 and 20 days post-larvae after metamorphosis. Thus, the results of the present study imply that Mnsxl1 and Mnsxl2 play complex and important roles in the sex differentiation of M. nipponense.