DNA damage in UVB-irradiated keratinocytes

A DNA lesion which results from the breakdown of a pyrimidine base leaving a formamido remnant has been associated with oxidative stress. This lesion is shown to be produced in keratinocytes irradiated in culture with UVB light. The amount of formamido lesion produced is comparable to the amount of...

Full description

Saved in:
Bibliographic Details
Published inCarcinogenesis (New York) Vol. 16; no. 7; p. 1659
Main Authors Maccubbin, A E, Przybyszewski, J, Evans, M S, Budzinski, E E, Patrzyc, H B, Kulesz-Martin, M, Box, H C
Format Journal Article
LanguageEnglish
Published England 01.07.1995
Subjects
Animals
Cell Line
DNA - metabolism
DNA - radiation effects
DNA Damage
Guanine - analogs & derivatives
Guanine - analysis
Guanine - metabolism
Keratinocytes - metabolism
Keratinocytes - radiation effects
Mice
Ultraviolet Rays
Online AccessGet more information

Cover

More Information
Summary:A DNA lesion which results from the breakdown of a pyrimidine base leaving a formamido remnant has been associated with oxidative stress. This lesion is shown to be produced in keratinocytes irradiated in culture with UVB light. The amount of formamido lesion produced is comparable to the amount of the 8-hydroxyguanine lesion. The two lesions were measured by 32P-postlabeling and electrochemical detection methods respectively.
ISSN:0143-3334
DOI:10.1093/carcin/16.7.1659