Resonance light scattering technique for the determination of proteins with resorcinol yellow and OP

A new resonance light scattering (RLS) assay of proteins is presented. In the citric acid–NaOH (pH 2.35) buffer, the RLS of Resorcinol yellow (RY)–protein system can be greatly enhanced by addition of nonionic surfactant OP, owing to the interaction between OP and RY–protein. The enhanced RLS is in...

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Published inTalanta (Oxford) Vol. 58; no. 5; pp. 869 - 874
Main Authors Chen, Yan-jing, Yang, Jing-he, Wu, Xia, Wu, Tao, Luan, Yu-xia
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 12.11.2002
Oxford Elsevier
Subjects
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Protein
Resonance light scattering (RLS)
Resorcinol yellow
OP
Resonance light scattering (RLS)
Resorcinol yellow
Protein
Vertebrata
Mammalia
Bovine
Hemoglobin
Biochemistry
Serum albumin
Artiodactyla
Ungulata
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Summary:A new resonance light scattering (RLS) assay of proteins is presented. In the citric acid–NaOH (pH 2.35) buffer, the RLS of Resorcinol yellow (RY)–protein system can be greatly enhanced by addition of nonionic surfactant OP, owing to the interaction between OP and RY–protein. The enhanced RLS is in proportion to the concentration of proteins in the range 0.02–4.0 μg ml −1 for both bovine serum albumin (BSA) and bovine hemoglobin (HEM), the detection limits were 10.4 ng ml −1 (S/N=3) for BSA and 11.4 ng ml −1 (S/N=3) for HEM. Samples were determined satisfactorily.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0039-9140
1873-3573
DOI:10.1016/S0039-9140(02)00401-0