Regulation of lymphocyte clustering by CD30-mediated ICAM-1 up-regulation

• Published in: Cellular immunology Vol. 219; no. 1; pp. 38 - 47
• Main Authors: Nam, Sang-Yun, Cho, Kyong-Shin, Heo, Young-Moon, Ha, Jong-Cheon, Kim, Young-Hyun, Keun Yi, Ho, Han Hwang, Pyong, Kim, Hyung-Min, Podack, Eckhard R
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.09.2002
• Subjects: Animals; Antigens, CD - metabolism; Apoptosis; Cell Adhesion Molecules - metabolism; Cell surface molecules; Cells, Cultured; Cellular activation; Costimulation; Cytokines - pharmacology; Intercellular Adhesion Molecule-1 - metabolism; Ki-1 Antigen - biosynthesis; Ki-1 Antigen - immunology; Lymph Nodes - cytology; Lymphocyte Function-Associated Antigen-1 - metabolism; Lymphocytes - metabolism; Mice; Mice, Inbred BALB C; NF-kappa B - pharmacology; Signal Transduction; Up-Regulation - drug effects; Cell surface molecules; Costimulation; Apoptosis; Cellular activation
• ISSN: 0008-8749; 1090-2163
• DOI: 10.1016/S0008-8749(02)00583-X

CD30 is expressed transiently on activated B and T lymphocytes and constitutively on several B- and T cell lymphomas. CD30 functions include participation in negative selection of thymocytes, costimulation of activated T cells, isotype switching of B cells, and regulation of the effector activity of cytotoxic lymphocytes. Although CD30 is not a marker for T helper 2 (TH2) cells, it may participate in the polarization of TH1 and TH2 cells. The pleiotropic functions of CD30 are initiated by interaction of CD30-expressing cells with other immune competent cells expressing CD30-L and providing the signals for modulation of effector cell activity. Here, we report that CD30 signals generated by anti-CD30 on activated, normal murine T cells strongly up-regulate the expression of intercellular adhesion molecule 1 (ICAM-1, CD54), and to a lesser extent, ICAM-2 (CD102). CD30 signals moreover delay the subsequent decline of ICAM expression. CD30 cross-linking did not alter the expression of CD11a/CD18 (LFA-1), the counter receptor for ICAM abundant on T cells. CD30-mediated ICAM-1 up-regulation is independent of cytokine secretion and appears to be transmitted directly through NF-κB activation. CD30-mediated up-regulation of ICAM-1 expression led to a significant increase in cluster formation of lymph node cells. Increased lymphocyte self-aggregation mediated by CD30 may set the stage for fraternal signaling to modulate lymphocyte function.