Electrochemical microwell sensor with Fe–N co-doped carbon catalyst to monitor nitric oxide release from endothelial cell spheroids

Endothelial cells have been widely used for vascular biology studies; recent progress in tissue engineering have offered three-dimensional (3D) culture systems for vascular endothelial cells which can be considered as physiologically relevant models. To facilitate the studies, we developed an electr...

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Published inAnalytical sciences Vol. 38; no. 10; pp. 1297 - 1304
Main Authors Hiramoto, Kaoru, Iwase, Kazuyuki, Utagawa, Yoshinobu, Nashimoto, Yuji, Honma, Itaru, Ino, Kosuke, Shiku, Hitoshi
Format Journal Article
LanguageEnglish
Published Singapore Springer Nature Singapore 01.10.2022
Subjects
Analytical Chemistry
Chemistry
Chemistry and Materials Science
Original Paper
Nitric Oxide (NO)
Composite carbon materials
Electrocatalysts
Endothelial cells
Spheroids
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Summary:Endothelial cells have been widely used for vascular biology studies; recent progress in tissue engineering have offered three-dimensional (3D) culture systems for vascular endothelial cells which can be considered as physiologically relevant models. To facilitate the studies, we developed an electrochemical device to detect nitric oxide (NO), a key molecule in the vasculature, for the evaluation of 3D cultured endothelial cells. Using an NO-sensitive catalyst composed of Fe–N co-doped reduced graphene oxide, the real-time monitoring of NO release from the endothelial cell spheroids was demonstrated. Graphical abstract
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ISSN:0910-6340
1348-2246
DOI:10.1007/s44211-022-00160-0