Circulating big human prolactin: conversion to small human prolactin by reduction of disulfide bonds

The heterogeneity of circulating human PRL (hPRL) in sera of patients with hyperprolactinemia was studied by exclusion chromatography on Sephadex G-100. Big hPRL, which elutes between the void volume and the elution position of monomeric small hPRL, is stable upon rechromatography but is almost enti...

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Published inThe journal of clinical endocrinology and metabolism Vol. 48; no. 5; p. 883
Main Authors Benveniste, R, Helman, J D, Orth, D N, McKenna, T J, Nicholson, W E, Rabinowitz, D
Format Journal Article
LanguageEnglish
Published United States 01.05.1979
Subjects
Adolescent
Adult
Chromatography, Gel
Disulfides
Female
Humans
Macromolecular Substances
Male
Oxidation-Reduction
Prolactin - blood
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Summary:The heterogeneity of circulating human PRL (hPRL) in sera of patients with hyperprolactinemia was studied by exclusion chromatography on Sephadex G-100. Big hPRL, which elutes between the void volume and the elution position of monomeric small hPRL, is stable upon rechromatography but is almost entirely converted into small hPRL after reduction with 0.5% mercaptoethanol. These results suggest that the existence of circulating big hPRL is dependent upon the formation of interpolypeptide disulfide bonds and does not represent a classical biosynthetic precursor of hPRL.
ISSN:0021-972X
DOI:10.1210/jcem-48-5-883