ATP induces leukemia inhibitory factor mRNA in cultured rat astrocytes

• Published in: Journal of neuroimmunology Vol. 129; no. 1; pp. 43 - 50
• Main Authors: Yamakuni, Hisashi, Kawaguchi, Nami, Ohtani, Yoshikazu, Nakamura, Junji, Katayama, Takahiro, Nakagawa, Takayuki, Minami, Masabumi, Satoh, Masamichi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2002
• Subjects: Adenosine Diphosphate - metabolism; Adenosine Diphosphate - pharmacology; Adenosine Triphosphate - analogs & derivatives; Adenosine Triphosphate - metabolism; Adenosine Triphosphate - pharmacology; Animals; Animals, Newborn; Astrocytes; Astrocytes - cytology; Astrocytes - drug effects; Astrocytes - metabolism; ATP; Cells, Cultured; Central Nervous System - cytology; Central Nervous System - growth & development; Central Nervous System - metabolism; Cytokine; Dose-Response Relationship, Drug; Fetus; Growth Inhibitors - genetics; Growth Inhibitors - metabolism; Interleukin-6; Leukemia Inhibitory Factor; Lymphokines - genetics; Lymphokines - metabolism; Microglia - cytology; Microglia - drug effects; Microglia - metabolism; Neurons - cytology; Neurons - drug effects; Neurons - metabolism; Neurotransmitter Agents - metabolism; Neurotransmitter Agents - pharmacology; Purinoceptor; Pyridoxal Phosphate - analogs & derivatives; Pyridoxal Phosphate - pharmacology; Rats; Rats, Sprague-Dawley; Receptors, Neurotransmitter - agonists; Receptors, Neurotransmitter - metabolism; Receptors, Purinergic - drug effects; Receptors, Purinergic - metabolism; Receptors, Purinergic P2 - drug effects; Receptors, Purinergic P2 - metabolism; Receptors, Purinergic P2Y2; RNA, Messenger - drug effects; RNA, Messenger - metabolism; Suramin - pharmacology; Uridine Triphosphate - metabolism; Uridine Triphosphate - pharmacology; UTP; UTP; Purinoceptor; 2-MeSATP; Astrocytes; Leukemia inhibitory factor; ATPγS; Cytokine; LIF; ADPβS; ATP; α,β-meATP; GFAP
• ISSN: 0165-5728; 1872-8421
• DOI: 10.1016/S0165-5728(02)00179-0

Leukemia inhibitory factor (LIF) is a cytokine involved in the survival and differentiation of the neural cells in the central and peripheral nervous systems. In the present study, we examined the effects of various neurotransmitter receptor agonists on LIF mRNA expression in cultured rat astrocytes, microglia and neurons to elucidate the cell types producing LIF and to clarify the neurotransmitter(s) regulating the mRNA expression. The results demonstrated that the expression of LIF mRNA was intensely induced by ATP in the cultured astrocytes. Experiments using ATP, UTP and related compounds showed the involvement of P2Y 2 and P2Y 4 purinoceptors in the expression induced by ATP.