Review: cyclodextrins and their interaction with amylolytic enzymes

• Published in: Enzyme and microbial technology Vol. 26; no. 8; pp. 561 - 567
• Main Authors: Hamilton, Lynn M, Kelly, Catherine T, Fogarty, William M
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.05.2000; Elsevier Science
• Subjects: Biological and medical sciences; Biotechnology; Enzyme engineering; Fundamental and applied biological sciences. Psychology; Improved methods for extraction and purification of enzymes; Methods. Procedures. Technologies; Glucan 1,4-α-glucosidase; Purification; Enzyme; Transferases; Glycosyltransferases; Review; α-Dextrin endo-1,6-α-glucosidase; β-Amylase; Cyclodextrin; Affinity chromatography; Glycosidases; Hydrolases; Cyclomaltodextrin glucanotransferase; Hexosyltransferases; Inhibition; α-Amylase; O-Glycosidases; Amylolytic enzyme
• ISSN: 0141-0229; 1879-0909
• DOI: 10.1016/S0141-0229(00)00141-1

This review is concerned with inhibition of amylases by cyclodextrins (cyclic maltooligosaccharides), the interaction that occurs between amylases and cyclodextrins and the application of cyclodextrin affinity chromatography in the purification of amylases. In many cases, amylases that are competitively inhibited by cyclodextrins can be purified by cyclodextrin affinity chromatography with the cyclodextrins interacting with the active site on such enzymes. Interestingly amylases that are not competitively inhibited by cyclodextrins may also be purified by cyclodextrin affinity chromatography. Therefore, cyclodextrin affinity chromatography can function in the purification of such amylolytic enzymes with the interaction occurring at a site removed from the active site. In such cases it appears that the cyclodextrin is interacting with an affinity site or binding site that is present on some amylolytic enzymes. It seems that certain similarities occur among the binding sites of such enzymes. Literature concerning amylases, and their subsequent purification using cyclodextrin affinity chromatography is reviewed and the fundamental basis of the interaction of the cyclodextrin with amylolytic enzymes is discussed here.