Allelic variation in the hemolymph juvenile hormone binding protein gene of Manduca sexta
Peripheral distribution of the insect juvenile hormones (JHs) requires hemolymph transport proteins. A comparison of three strains of the tobacco hornworm Manduca sexta indicates that hemolymph JH binding protein (hJHBP) levels in the Madison wild-type ( Mwt) strain are significantly higher than in...
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Published in | Molecular and cellular endocrinology Vol. 208; no. 1; pp. 41 - 50 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Ireland
Elsevier Ireland Ltd
31.10.2003
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Subjects | |
Online Access | Get full text |
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Summary: | Peripheral distribution of the insect juvenile hormones (JHs) requires hemolymph transport proteins. A comparison of three strains of the tobacco hornworm
Manduca sexta indicates that hemolymph JH binding protein (hJHBP) levels in the Madison wild-type (
Mwt) strain are significantly higher than in the black larval mutant (
bl) and Seattle wild-type (
Swt) strains. To correlate differences in hJHBP levels between strain phenotypes with the hJHBP locus, we sequenced 8.4
kb of the hJHBP gene locus from each strain.
Snb, an allele found in the
Swt and
bl strains, contains a 408
bp repetitive nuclear element flanked by 15
bp direct repeats. Mating studies coupled with molecular genotyping demonstrate that the presence of
Snb correlates with a twofold lower hJHBP level relative to the allele found in the
Mwt strain. Despite the lower hJHBP levels in individuals carrying the
Snb gene, hemolymph levels of JH do not appear to be significantly affected. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0303-7207 1872-8057 |
DOI: | 10.1016/S0303-7207(03)00259-4 |