Influence of culture conditions for clinically isolated non-albicans Candida biofilm formation

• Published in: Journal of microbiological methods Vol. 130; pp. 123 - 128
• Main Authors: Tan, Yulong, Leonhard, Matthias, Ma, Su, Schneider-Stickler, Berit
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2016
• Subjects: Batch Cell Culture Techniques - methods; Biofilm; Biofilms - growth & development; Candida - classification; Candida - growth & development; Candida - isolation & purification; Candida - metabolism; Candida krusei; Candida parapsilosis; Candida tropicalis; Cell Adhesion; Clinical isolates; Culture Media - chemistry; Humans; Larynx, Artificial - microbiology; Non-albicans Candida species; Biofilm; Clinical isolates; Non-albicans Candida species
• ISSN: 0167-7012; 1872-8359
• DOI: 10.1016/j.mimet.2016.09.011

Non-albicans Candida species have been isolated in increasing numbers in patients. Moreover, they are adept at forming biofilms. This study analyzed biofilm formation of clinically isolated non-albicans Candida, including Candida tropicalis, Candida krusei and Candida parapsilosis under the influence of different growth media (RPMI 1640, YPD and BHI) and several culture variables (inoculum concentration, incubation period and feeding conditions). The results showed that culture conditions strongly influenced non-albicans Candida species biofilm formation. YPD and BHI resulted in larger amount of biofilm formation with higher metabolic activity of biofilms. Furthermore, the growth media seems to have varying effects on adhesion and biofilm development. Growth conditions may also influence biofilm formation, which was enhanced when starting the culture with a larger inoculum, longer incubation period and using a fed-batch system. Therefore, the potential influences of external environmental factors should be considered when studying the non-albicans Candida biofilms in vitro. •Culture conditions influenced non-albicans Candida species biofilm formation.•YPD and BHI resulted in larger biofilm formation with stronger metabolic activity.•The growth media had varying effects on the adhesion and biofilm development.•Higher inoculum, longer incubation period and fed-batch system enhanced biofilm.