Spectrophotometric determination of iron with ferrozine by flow-injection analysis

• Published in: Talanta (Oxford) Vol. 44; no. 10; pp. 1793 - 1801
• Main Authors: PASCUALREGUERA, M, ORTEGACARMONA, I, MOLINADIAZ, A
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.10.1997; Oxford Elsevier
• Subjects: Analytical chemistry; Analytical, structural and metabolic biochemistry; Applied sciences; Biological and medical sciences; Chemistry; Exact sciences and technology; Ferrozine; Flow system; Fundamental and applied biological sciences. Psychology; Inorganic compounds; Iron; Metals. Metallurgy; Other biological molecules; Spectrometric and optical methods; Spectrophotometry; Flow system; Iron; Spectrophotometry; Ferrozine; Trace analysis; Plants (botany); Chemical analysis; Wastewater; Biological materials; Sulfonate; Bauxite; Iron complex; Triazine derivatives; Flow injection; Ultraviolet visible spectrometry
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/S0039-9140(97)00050-7

Two methods for the determination of iron by normal FIA and reversed FIA were developed using sodium 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine-4′,4″-disulphonate (ferrozine). The reagent formed a chelate with Fe(II) in hexamethylentetramine buffered medium at pH 5.5. In one previous reaction coil Fe(III) was reduced to Fe(II) by ascorbic acid and in the other reaction coil the complexation reaction was developed. The linear range of the determination was 0.5–6 and 0.1–5 μg ml −1 of iron for normal FIA and reversed FIA respectively. The proposed method was sensitive (detection limit 0.012 and 0.010 μg ml −1), rapid and reproducible (RSD 0.3 and 0.28%). The method was satisfactorily applied to the determination of iron in waste water, toadstool tissue, potato leaves, human hair and bauxites at a sampling rate of 90 and 50 samples h −1 for normal FIA and reversed FIA respectively.