Fibre Optic Reflection Photometry on Blood

A two photocell assembly has been used to measure the light reflection of blood conducted by fibre optics. Fibre optic reflection spectra of oxygenated and reduced blood samples show an isobestic region around 850 nm. Non-specific variations of the light reflection from a mirror do not affect the ra...

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Bibliographic Details
Published inCardiovascular research Vol. 2; no. 2; pp. 199 - 209
Main Authors Mook, Gerrit A., Osypka, Peter, Sturm, Ralph E., Wood, Earl H.
Format Journal Article
LanguageEnglish
Published England Oxford University Press 01.04.1968
Subjects
Animals
Blood Chemical Analysis
Catheterization
Coloring Agents - blood
Densitometry
Dogs
Dye Dilution Technique
Hemoglobinometry
Light
Methods
Optics and Photonics
Oximetry
Photometry - instrumentation
Swine
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Summary:A two photocell assembly has been used to measure the light reflection of blood conducted by fibre optics. Fibre optic reflection spectra of oxygenated and reduced blood samples show an isobestic region around 850 nm. Non-specific variations of the light reflection from a mirror do not affect the ratio of detecting and compensating photocell outputs, whereas the ratio is not independent of variations in blood flow and haemoglobin concentration. The relation of log R640/R800 and the oxygen saturation is linear. The relation of R900/R800 and indocyanine green dye concentration is linear up to 20 mg/l.
Bibliography:This investigation was supported in part by Research Grant H-3532 from the National Institutes of Health, Bethesda.
On leave from September 1963 to September 1964 from the Laboratory of Chemical Physiology, University of Groningen, the Netherlands.
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ArticleID:2-2-199
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content type line 23
ISSN:0008-6363
1755-3245
DOI:10.1093/cvr/2.2.199