A simple method for the study of salbutamol pharmacokinetics by ion chromatography with direct conductivity detection

• Published in: Talanta (Oxford) Vol. 65; no. 1; pp. 1 - 6
• Main Authors: Ouyang, Jin, Duan, Jing Li, Baeyens, Willy R.G., Delanghe, Joris R.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.01.2005; Oxford Elsevier
• Subjects: Analytical chemistry; Biological and medical sciences; Chemistry; Chromatographic methods and physical methods associated with chromatography; Electrochemical methods; Exact sciences and technology; General pharmacology; Ion chromatography; Medical sciences; Other chromatographic methods; Pharmacokinetics; Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions; Pharmacology. Drug treatments; Salbutamol; Pharmacokinetics; Ion chromatography; Salbutamol; Fluorescence detector; Linear regression; Internal standard; HPLC chromatography; Mobile phase; Nitric acid; Regression coefficient; Reversed phase chromatography; Chemical modification; Conductometry; Detection limit; Acetonitrile; Standard curve; Signal to noise ratio
• ISSN: 0039-9140; 1873-3573; 1873-3573
• DOI: 10.1016/j.talanta.2004.01.026

A simple method for the study of the pharmacokinetics of salbutamol using ion chromatography with direct conductivity detection without chemical suppression is presented in this paper. Baseline separation of salbutamol from plasma components was achieved by using diluted HNO 3 (2 mmol l −1) as mobile phase with acetonitrile (6%, v/v) as modifier. Atenolol was utilized as internal standard. Under the optimal conditions, the linear regression coefficients of the calibration curves are 0.996 within the concentration range of 1000–3 ng ml −1 salbutamol. The detection limit (signal-to-noise ratio = 3) was 1 ng ml −1 salbutamol. The noncompartmental pharmacokinetic parameters for salbutamol following administration of 8 mg salbutamol to 18 subjects were tested. The results coincided most satisfactorily with the results obtained by using reversed-phase high performance liquid chromatography (HPLC) with fluorescence detection.