Induction of CD28 on the new myeloma cell line MOLP-8 with t(11;14)(q13;q32) expressing δ/λ type immunoglobulin

• Published in: Leukemia research Vol. 28; no. 8; pp. 869 - 877
• Main Authors: Matsuo, Yoshinobu, Drexler, Hans G, Harashima, Akira, Okochi, Ayumi, Hasegawa, Atsuhiko, Kojima, Kensuke, Orita, Kunzo
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.08.2004
• Subjects: Antigens, CD - metabolism; Antigens, Surface - metabolism; CD28; CD28 Antigens - metabolism; Cell Adhesion; Cell Division; Cell line; Chromosomes, Human, Pair 11 - genetics; Chromosomes, Human, Pair 14 - genetics; Humans; Immunoglobulin delta-Chains - metabolism; Immunoglobulin lambda-Chains - metabolism; Integrins - metabolism; Karyotyping; Male; Middle Aged; Multiple myeloma; Multiple Myeloma - genetics; Multiple Myeloma - immunology; Multiple Myeloma - pathology; Plasma Cells - pathology; Stromal Cells - metabolism; Stromal Cells - pathology; Translocation, Genetic - genetics; Tumor Cells, Cultured; Vascular Cell Adhesion Molecule-1 - metabolism; Japan; CD28; Cell line; Cell adhesion; Multiple myeloma
• ISSN: 0145-2126; 1873-5835
• DOI: 10.1016/j.leukres.2003.12.008

The novel multiple myeloma (MM) cell line MOLP-8 carrying the t(11;14) (q13;q32) was established from the peripheral blood of a 52-year-old Japanese male patient with Bence–Jones δ/λ type MM (stage IIIA with hyperammonemia). The growth of MOLP-8 cells is constitutively independent of exogenous growth factors or feeder cells. MOLP-8 cells grow mainly as free floating single cells and slightly adherent on the bottom of the plastic culture flask. Wright–Giemsa-stained MOLP-8 cells show the typical plasma cell morphology with abundant cytoplasm, heterogeneous cell size and one to three nuclei. The immunoprofile of MOLP-8 corresponds to that seen typically in primary MM cells: positive for cytoplasmic immunoglobulin (Ig) δ/λ chains, CD10, CD29, CD38, CD40, CD44, CD49b, CD49d, CD54, CD56, CD58, CD71, CD138 and PCA-1; the cells were negative for surface Igs and various other B-cell, T-cell and myelomonocyte-associated immunomarkers. CD28 became positive after co-culture of MOLP-8 cells with bone marrow adherent stromal (BST) feeder cells for a week. About 30% of MOLP-8 cells adhered strongly to the BST cells, but the cellular adhesion was clearly inhibited by addition of either anti-CD29 or anti-CD106 monoclonal antibody, suggesting a specific cellular adhesion through α4β1-integrin–VCAM-1 interaction. The novel MOLP-8 cell line together with the present myeloma cell lines will present useful model systems in the investigation of the biology of MM.